PJA1 抗体 (AA 50-100)
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- 抗原 See all PJA1 抗体
- PJA1 (Praja Ring Finger 1, E3 Ubiquitin Protein Ligase (PJA1))
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抗原表位
- AA 50-100
- 适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PJA1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
- 特异性
- PJA1 antibody is human, mouse and rat reactive. At least two isoforms of PJA1 are known to exist, this antibody will detect both isoforms.
- 纯化方法
- PJA1 antibody is affinity chromatography purified via peptide column.
- 免疫原
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PJA1 antibody was raised against a 19 amino acid peptide near the amino terminus of human PJA1.
The immunogen is located within amino acids 50 - 100 of PJA1. - 亚型
- IgG
- Top Product
- Discover our top product PJA1 Primary Antibody
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anti-Praja Ring Finger 1, E3 Ubiquitin Protein Ligase (PJA1) (AA 81-180) antibody
PJA1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Praja Ring Finger 1, E3 Ubiquitin Protein Ligase (PJA1) (AA 81-180) antibodyPJA1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3E10 unconjugated
anti-Praja Ring Finger 1, E3 Ubiquitin Protein Ligase (PJA1) (AA 154-183), (N-Term) antibodyPJA1 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- PJA1 antibody can be used for detection of PJA1 by Western blot at 0.25 - 0.5 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PJA1 antibody is supplied in PBS containing 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- PJA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
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- 抗原
- PJA1 (Praja Ring Finger 1, E3 Ubiquitin Protein Ligase (PJA1))
- 别名
- PJA1 (PJA1 产品)
- 别名
- PRAJA1 antibody, RNF70 antibody, praja ring finger ubiquitin ligase 1 antibody, PJA1 antibody, Pja1 antibody
- 背景
- Ubiquitinization is an important cellular degradation process requiring sequential reactions that are mediated by three enzymes: E1, E2 and E3. PJA1, also known as Praja1 and RING finger protein 70, is a 643 amino acid E2-dependent E3-ubiquitin ligase that is abundantly expressed in the brain (1,2). Through interaction and activation with the E2-ubiquitin ligase UBC4, PJA1 mediates substrate-specific ubiquitination via its RING finger domain and facilitates ubiquitination (3). Overexpression of PJA1 in gastrointestinal cancers suggests that it may be responsible for the degradation of some anti-oncogenic proteins (4,5).
- 分子量
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Predicted: 71 kDa
Observed: 71 kDa - 基因ID
- 64219
- NCBI登录号
- NP_660095
- UniProt
- Q8NG27
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