Profilin 抗体
PFN
适用: 人, Cow, 小鼠, Pig, 大鼠
WB, ICC, IHC
宿主: 兔
Polyclonal
unconjugated
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- 抗原 See all Profilin (PFN) 抗体
- Profilin (PFN)
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适用
- 人, Cow, 小鼠, Pig, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Profilin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
- 特异性
- Recognizes human, mouse, rat, pig, bovine and marsupial profilin.
- 纯化方法
- Antigen affinity purified.
- 免疫原
- Human full-length profilin.
- Top Product
- Discover our top product PFN Primary Antibody
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anti-Profilin (PFN) (AA 1-133) antibody
PFN 适用: Mercenaria (Clam) ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Profilin (PFN) antibodyPFN 适用: 人, Cow, 豚鼠, 兔 WB, ICC, IHC 宿主: 小鼠 Monoclonal 2H11 unconjugated
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- 应用备注
- ELISA: (direct or indirect: 1:2,000-1:5,000). Western blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05 % Tween 20 and 5 % skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 100 μg/mL
- 缓冲液
- Liquid. In PBS containing 1 mg/mL BSA and 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Stable for at least 1 year after receipt when stored at -20°C.
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- 抗原
- Profilin (PFN)
- 别名
- Profilin (PFN 产品)
- 别名
- LOC406154 antibody, GB13380 antibody, profilin antibody, PFN antibody, PRF antibody
- 背景
- Profilin (PFN1) is a ubiquitous small (12-15 kDa) phosphoinositide and poly-L-proline binding protein that plays a role in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels also in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes. Profilin binding to PIP2 interferes with PIP2 hydrolysis by soluble phospholipase C-gamma, an inhibition that can be overcome by tyrosine phosphorylation of PLC-gamma. Besides actin monomer sequestration and stimulation of actin nucleotide exchange, profilin can also promote cellular actin filament growth. Profilin is involved in the actin dependent intracellular motility of cytopathogenic bacteria, the regulation of cell adhesion and possibly also in linking the actin cytoskeleton and endocytosis. Profilin has been found to associate with defined complexes containing proteins such as Arp2/3 or the Rho/Rac pathways constituents ROCK-II and HEM2/NAP1. Defects in PFN1 are the cause of amyotrophic lateral sclerosis 18 (ALS18).
- 基因ID
- 5216
- NCBI登录号
- NP_005013
- UniProt
- P07737
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