G-CSF 抗体
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北京 101111
Quick Overview for G-CSF 抗体 (ABIN5689134)
抗原
See all G-CSF (CSF3) 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Anti-mG-CSF specific antibody was purified by affinity chromatography employing immobilized mG-CSF matrix.
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免疫原
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mG-CSF.
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应用备注
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mG-CSF (0.5 ng/mL), a concentration of 0.005 - 0.010 μ,g/mL of this antibody is required.
ELISA:
To detect mG-CSF by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mG-CSF.
Sandwich: To detect mG-CSF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine G-CSF (XP-5142Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mG-CSF.
Western Blot:
To detect mG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mG-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- -20 °C
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储存方法
- G-CSF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
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别名
- G-CSF
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基因ID
- 12985
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UniProt
- P09920
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
抗原
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