Western Blotting (WB), Immunohistochemistry (IHC), ELISA
特异性
Recognizes the fibrinogen-like domain (FLD) of human ANGPTL4. Detects a band of ~62 kDa and ~35 kDa by Western blot. Does not cross-react with other ANGPTL family proteins.
ANGPTL4
适用: 人, 小鼠
WB, IF
宿主: 兔
Polyclonal
unconjugated
应用备注
ELISA: (direct or indirect: 1:2,000-1:5,000). Immunohistochemistry: (paraffin sections (1:100-1:1,000)). Western blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05 % Tween 20 and 5 % skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions should be determined individually for each application.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
储存液
Without preservative
储存条件
-20 °C
储存方法
Stable for at least 1 year after receipt when stored at -20°C.
ANGPTL4 mainly expressed in endothelial cells (hypoxia-induced). Regulates angiogenesis and modulates tumorgenesis and directly regulates lipid, glucose, and energy metabolism. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. ANGPTL4 is a protein consisting of an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain (FLD). Both domains have distinct biological functions. The coiled-coil domain is responsible for the inhibitory effects on lipoprotein lipase (LPL) converting the active form of LPL into an inactive form, and the FLD domain mediates its antiangiogenic functions. The coiled coil and the FLD domains are separated by a short linker that can be cleaved after secretion. ANGPTL4 appears on the cell surface as the full-length form, where it can be released by heparin treatment. ANGPTL4 protein is then proteolytically cleaved by proprotein convertases (PCs), including furin, PC5/6, paired basic amino acid-cleaving enzyme 4, and PC7.