ELISA: (direct or indirect: 1:2,000-1:10,000). Flow Cytometry: (1:100-1:500). Immunohistochemistry: (paraffin sections (1:100-1:500)). Western blot: (1:2,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05 % Tween 20 and 5 % skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
储存液
Without preservative
储存条件
-20 °C
储存方法
Stable for at least 1 year after receipt when stored at -20°C.
DLK1 (Protein delta homolog 1, Preadipocyte factor 1, Pref-1) is a regulator of adipocyte differentiation found in serum and urine. DLK1 might be a decoy ligand. Serum or plasma levels of soluble DLK1 affect negatively or positively adipogenesis and control mesenchymal cell fate. DLK1 is frequently upregulated in myelodysplastic syndrome (MDS) patients compared to non-leukemic individuals.