This antibody is expected to recognize all isoforms (NP_001123952.1, NP_001123954.1, NP_001123955.1, NP_001129499.1). Reported variants represent identical protein: NP_001123953.1, NP_006287.2.
纯化方法
VRK2 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
VRK2 Antibody was raised against synthetic peptide PNSQKVDSQKAATK found at an internal region of VRK2.
VRK2
适用: 小鼠
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
Alexa Fluor 488
应用备注
ELISA: antibody detection limit dilution 1:4000. Western Blot: Approx 60 kDa band observed in nuclear lysates of cell line K562 (calculated MW of 58.1 kDa according to NP_001123954.1). Recommended concentration: 1-3μ,g/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
VRK2 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Aliquot and store at -20°C. Minimize freezing and thawing.