Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
特异性
This antibody is expected to recognize both reported isoforms (NP_003783.1, NP_694880.1).
纯化方法
EDF1 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
EDF1 Antibody was raised against synthetic peptide NKQHSITKNTAKLDR found at an internal region of EDF1.
ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 17 kDa band observed in lysates of cell line A549 (calculated MW of 16.4 kDa according to NP_003783.1). Recommended concentration: 0.3-1μ,g/mL. Immunohistochemistry: In paraffin embedded Human Spleen shows nuclear staining of select lymphocytes in the marginal zone. Recommended concentration, 5-10μ,g/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
EDF1 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Aliquot and store at -20°C. Minimize freezing and thawing.
抗原
EDF1
(Endothelial Differentiation Related Factor 1 (EDF1))