This antibody is expected to recognize both reported isoforms (NP_001164645.1, NP_001328.1). Reported variants represent identical protein: NP_001164643.1, NP_001328.1, NP_001164642.1.
纯化方法
CX3CR1 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
CX3CR1 Antibody was raised against synthetic peptide DQFPESVTENFEYD found at an internal region (near the N Terminus) of CX3CR1.
ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 39 kDa band observed in lysates of cell line Kelly and 37 kDa in lysates of cell line U251-MG (calculated MW of 40.4 kDa according to NP_001328.1). Recommended concentration: 1-3μ,g/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
CX3CR1 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Aliquot and store at -20°C. Minimize freezing and thawing.