This antibody is expected to recognize both reported isoforms (NP_001190.1, NP_006120.1).
纯化方法
BMP1 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
BMP1 Antibody was raised against synthetic peptide DTIVPKYEVNGVK found at an internal region of BMP1.
ELISA: antibody detection limit dilution 1:128000. Western Blot: 1μ,g/mL staining of Human Heart and Kidney lysates, 2μ,g/mL staining of Rat Heart lysate, and 2μ,g/mL staining of NIH3T3 cell lysate (35μ,g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
BMP1 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Aliquot and store at -20°C. Minimize freezing and thawing.