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IL1A 抗体

This 山羊 多克隆 antibody specifically detects IL1A in WB, ELISA 和 Neut. It exhibits reactivity toward 大鼠.
产品编号 ABIN5687646
发货至: 中国
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北京 101111
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for IL1A 抗体 (ABIN5687646)

抗原

See all IL1A 抗体
IL1A (Interleukin 1 alpha (IL1A))

适用

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大鼠

宿主

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山羊

克隆类型

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多克隆

标记

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This IL1A antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Neutralization (Neut)
  • 纯化方法

    Anti-Rat IL-1alpha specific antibody was purified by affinity chromatography employing immobilized Rat IL-1alpha matrix.

    免疫原

    Produced from sera of goats pre-immunized with highly pure (>98%) recombinant Rat IL-1alpha (Rat IL-1alpha).
  • 应用备注

    Neutralization:
    To yield one-half maximal inhibition [ND50] of the biological activity of Rat IL-1α, (50pg/mL), a concentration of 0.011-0.017 μ,g/mL of this antibody is required.

    ELISA:

    To detect Rat IL-1α, by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat IL-1α,.

    Sandwich:

    To detect Rat IL-1α, by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat IL-1α, (XP-5176Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-1α,.

    Western Blot:
    To detect Rat IL-1α, by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-1α, is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    限制

    仅限研究用
  • 状态

    Lyophilized

    储存条件

    -20 °C

    储存方法

    IL-1a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • 抗原

    IL1A (Interleukin 1 alpha (IL1A))

    别名

    IL-1a

    基因ID

    24493

    UniProt

    P16598

    途径

    NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
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