IGF1 抗体
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北京 101111
Quick Overview for IGF1 抗体 (ABIN5687643)
抗原
See all IGF1 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Anti-murine IGF-I specific antibody was purified by affinity chromatography employing immobilized murine IGF-I matrix.
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免疫原
- Produced from sera of goats pre-immunized with highly pure (>98%) recombinant murine IGF-I.
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anti-Insulin-Like Growth Factor 1 (IGF1) (AA 49-118) antibody
IGF1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) (AA 49-118) antibodyIGF1 适用: 人 WB, ICC, IF 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) (AA 49-118) antibodyIGF1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) (AA 49-153) antibodyIGF1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) (Internal Region) antibodyVerified IGF1 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) (AA 115-144) antibodyIGF1 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) (AA 49-100) antibodyIGF1 适用: 人, 小鼠, 大鼠, 犬 WB, IP, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) (AA 33-102) antibodyIGF1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) (AA 115-144), (C-Term) antibodyIGF1 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB33711 unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) (AA 50-150) antibodyIGF1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of murine IGF-I (200 ng/mL), a concentration of 12-14 μ,g/mL of this antibody is required.
ELISA:
To detect murine IGF-I by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant murine IGF-I.
Sandwich:
To detect mIGF-I by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine IGF-I (XP-5159Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIGF-I.
Western Blot:
To detect murine IGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant murine IGF-I is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- -20 °C
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储存方法
- IGF-I antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- IGF1 (Insulin-Like Growth Factor 1 (IGF1))
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别名
- IGF-I
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基因ID
- 16000
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UniProt
- P05017
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途径
- RTK signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation
抗原
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