IGF1 抗体 (Biotin)
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北京 101111
Quick Overview for IGF1 抗体 (Biotin) (ABIN5687628)
抗原
See all IGF1 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Anti-mIGF-I specific antibody was purified by affinity chromatography and then biotinylated.
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免疫原
- Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mIGF-I (murine IGF-I).
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应用备注
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ELISA:
To detect mIGF-I by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant mIGF-I.
Sandwich
To detect mIGF-I by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine IGF-I (XP-5159) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIGF-I.
Western Blot:
To detect mIGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIGF-I is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- -20 °C
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储存方法
- IGF-I antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- IGF1 (Insulin-Like Growth Factor 1 (IGF1))
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别名
- IGF-I
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基因ID
- 16000
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UniProt
- P05017
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途径
- RTK signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation
抗原
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