Western Blotting (WB), ELISA, Immunofluorescence (IF)
纯化方法
ACAT1 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
ACAT1 Antibody was raised against synthetic peptide DEEYKRVDFSKVPK found at an internal region of ACAT1.
ELISA: antibody detection limit dilution 1:2000. Western Blot: Approx 45 kDa band observed in Human, Mouse and Rat Liver lysates (calculated MW of 45.2 kDa according to Human NP_000010.1, 44.8 kDa according to Mouse NP_659033.1 and 44.7 kDa according to Rat NP_058771.2). Recommended concentration: 0.1-0.3μ, Immunofluorescence:Customer finds particulate cytoplasm staining in HeLa.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
ACAT1 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Aliquot and store at -20°C. Minimize freezing and thawing.