Tested in Immunoelectrophoresis and Double Radial Immunodiffusion against normal Human serum, a single precipitin line is obtained which gives a reaction of full identity with the isolated immunogen. The product does not react with any other proteins component of Human serum or plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
The IgG fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by Immunoelectrophoresis and double Radial Immunodiffusion.
免疫原
The subunit C1q is isolated as a homogenous protein for use in antiserum production. Freund’s complete adjuvant is used in the first step of the immunization.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
状态
Liquid
溶解方式
Restore by adding 1 mL of sterile distilled water.
浓度
10.8 mg/mL
缓冲液
PBS, pH 7.2 without preservatives.
储存液
Without preservative
注意事项
Avoid Repeated thawing and freezing.
储存条件
4 °C/-20 °C
储存方法
Store lyophilized at 2-8 °C and reconstituted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
抗原
C1QA
(Complement Component 1, Q Subcomponent, A Chain (C1QA))
别名
C1q A Subunit
背景
C1 component of human complement is a complex of three glycoproteins: C1q, C1r and C1s. C1q has a molecular weight of 492.000 and is present in plasma in an average concentration of 80 mg/mL. It consists of a complex of 18 polypeptide chains of three different types, A, B and C, 6 of each type. The subcomponent C1q is responsible for the binding of C1q to immuno-complexes an membranes via the Fc portion of the immunoglobulin.Synonyms: Complement 1q, Complement C1q