Collagen Type I 抗体
Quick Overview for Collagen Type I 抗体 (ABIN5680217)
抗原
See all Collagen Type I (COL1) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Mouse collagen type I 100 % , mouse collagen type II and IV <0.1 %, mouse collage type III <1 %, Chicken and rat collagen type I < 0.1 % (RIA at 1:200 dilution)
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交叉反应 （详细）
- Mouse, human, chicken and rat collagen type I < 0.1 % (RIA at 1:200 dilution)
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产品特性
- Type I Collagen usually exists as a heterotrimer formed by alpha 1(I) and alpha 2(I) chains and is found in bone, cornea, skin and tendon. In foetal tissues also homotrimers of alpha-1(I) are found, but they are not constituents of normal adult tissues. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Mouse collagen type I 100 % , mouse collagen type II and IV <0.1 %, mouse collage type III <1 %, .
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纯化方法
- Affinity purified
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免疫原
- Purified collagen type I from murine skin
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亚型
- IgG
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anti-Collagen, Type I (COL1) antibody
COL1 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type I (COL1) (AA 1321-1400) antibodyCOL1 适用: 人, 大鼠, 小鼠, 兔, 犬, 鱼 WB, ELISA, IHC (fro), IHC (p), IF (p), IF (cc), FACS 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type I (COL1) (AA 1051-1150) antibodyCOL1 适用: 人, 大鼠, 小鼠, 兔 WB, ELISA, IHC (fro), IHC (p), IF (p), ICC, IF (cc), FACS 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type I (COL1) antibodyCOL1 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 小鼠 Monoclonal 3G3 unconjugated
anti-Collagen, Type I (COL1) antibody (Biotin)COL1 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin
anti-Collagen, Type I (COL1) antibodyCOL1 适用: 人, 小鼠, 哺乳动物, 鱼, Amphibian, Avian WB, IHC (fro), IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type I (COL1) antibodyCOL1 适用: 人 ELISA 宿主: 小鼠 Monoclonal 4F6 unconjugated
anti-Collagen, Type I (COL1) antibody (Biotin)COL1 适用: 人 ELISA, FLISA 宿主: 小鼠 Monoclonal 4F6 Biotin
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应用备注
- After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Mouse skin,IHC(P), IFA, ELISA, RIA, IB/WB,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:500, ELISA ? 1:200 (OD ? 500)
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限制
- 仅限研究用
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状态
- Lyophilized
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浓度
- 1 mg/mL
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缓冲液
- phosphate buffered solution, no BSA and preservative added!
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储存液
- Without preservative
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储存条件
- -20 °C
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- Collagen Type I (COL1) (Collagen, Type I (COL1))
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别名
- Collagen Type I
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UniProt
- P11087
抗原
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