Collagen Type I 抗体
(3 references)-
- 抗原 See all Collagen Type I (COL1) 抗体
- Collagen Type I (COL1) (Collagen, Type I (COL1))
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适用
- 人, 小鼠, 哺乳动物, 鱼, Amphibian, Avian
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Collagen Type I antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
- 特异性
- Specific for the ~180 kDa Collagen I Alpha-1 Propeptide in Human lung fibroblast extract.
- 交叉反应 （详细）
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Species reactivity (expected):most (if not all) Species of Mammals, Birds, Amphibians and Fishes.
Species reactivity (tested):Human and Mouse. - 纯化方法
- Affinity Chromatography.
- 免疫原
- Peptide from the Human Collagen I Alpha-1 propeptide sequence.
- 亚型
- IgG
- Top Product
- Discover our top product COL1 Primary Antibody
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anti-Collagen, Type I (COL1) antibody
COL1 适用: 人, 大鼠, 小鼠, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type I (COL1) antibodyCOL1 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type I (COL1) (AA 1321-1400) antibodyCOL1 适用: 人, 大鼠, 小鼠, 兔, 犬, 鱼 WB, ELISA, IHC (fro), IHC (p), IF (p), IF (cc), FACS 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type I (COL1) (AA 1051-1150) antibodyCOL1 适用: 人, 大鼠, 小鼠, 兔 WB, ELISA, IHC (fro), IHC (p), IF (p), IF (cc), ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type I (COL1) antibody (FITC)COL1 适用: 人, 大鼠, 小鼠, Cow, 哺乳动物 WB, IHC, IP, FACS 宿主: 兔 Polyclonal FITC
anti-Collagen, Type I (COL1) antibody (Biotin)COL1 适用: 人, 大鼠, 小鼠, Cow, 哺乳动物 WB, ELISA, IHC, IP, FACS 宿主: 兔 Polyclonal Biotin
anti-Collagen, Type I (COL1) antibody (HRP)COL1 适用: 人, 大鼠, 小鼠, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal HRP
anti-Collagen, Type I (COL1) antibodyCOL1 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 小鼠 Monoclonal 3G3 unconjugated
anti-Collagen, Type I (COL1) antibodyCOL1 适用: 人, 大鼠, 小鼠 IHC (p), IF 宿主: 小鼠 Monoclonal 4H10 unconjugated
anti-Collagen, Type I (COL1) antibody (Biotin)COL1 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin
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- 应用备注
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Western Blot: 1/1000. Immunohistochemitry: 1/100. The antibody also works well for Immunohistochemistry on Paraformaldehyde-FixedSections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 °C in10 mM sodium citrate ( pH 6.0)/ 0.1 % Tween-20). Note that in Paraffin Sections of Formaldehyde-Fixed fibrotic mouse lung tissue, itrecognizes collagen I molecules that are still associated with the cells in which they weresynthesized.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Liquid in PBS. Contains no preservative
- 储存液
- Without preservative
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store the antibody undiluted (in aliquots) at-20 °C.
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: "Effects of chronically increased VEGF-A on the aging heart." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 32, Issue 3, pp. 1550-1565, (2018) (PubMed).
: "In Vitro Identification of New Transcriptomic and miRNomic Profiles Associated with Pulmonary Fibrosis Induced by High Doses Everolimus: Looking for New Pathogenetic Markers and Therapeutic Targets." in: International journal of molecular sciences, Vol. 19, Issue 4, (2018) (PubMed).
: "Hypoxia Inducible Factors Modify Collagen I Fibers in MDA-MB-231 Triple Negative Breast Cancer Xenografts." in: Neoplasia (New York, N.Y.), Vol. 20, Issue 2, pp. 131-139, (2018) (PubMed).
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: "Effects of chronically increased VEGF-A on the aging heart." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 32, Issue 3, pp. 1550-1565, (2018) (PubMed).
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- 抗原
- Collagen Type I (COL1) (Collagen, Type I (COL1))
- 别名
- Collagen Type I (COL1 产品)
- 别名
- collagen type III alpha 1 chain antibody, COL3A1 antibody
- 背景
- Collagen is the most abundant protein in mammals with type 1 collagen being the most abundant of the 29 known collagens. Type 1 collagen is integral in the strengthening and structural support of many tissues including skin, tendon, artery walls, fibrocartilage, bone and teeth. Collagens are synthesized with N-terminal and C-terminal propeptides that are cleaved during maturation and secretion. After cleavage of the propeptides, the most N-terminal and C-terminal remaining sequences are known as telopeptides. Mutations in the collagen 1, alpha 1 gene (COL1A1) are known to cause osteogenesis imperfecta (aka brittle bone disease) (Byers 1989). Furthermore, mutations found in the fist 90 residues of the helical region of alpha 1 collagen have been implicated in the prevention or delayed removal of the procoallagen N-propeptide leading to a combined osteogenesis imperfecta and Ehlers-Danlos syndrome (EDS) phenotype (Cabral et al., 2005).Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2
- 基因ID
- 1277
- NCBI登录号
- NP_000079
- UniProt
- P02452
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