Collagen Type I 抗体
Quick Overview for Collagen Type I 抗体 (ABIN5680210)
抗原
See all Collagen Type I (COL1) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- Tunafish collagen type I Crossreactivity with collagen type I of other species <0.1 %
-
交叉反应 (详细)
- Tunafish, crossreactivity with collagen type I of other species <0.1 %
-
产品特性
- Type I Collagen usually exists as a heterotrimer formed by alpha 1(I) and alpha 2(I) chains and is found in bone, cornea, skin and tendon. In foetal tissues also homotrimers of alpha-1(I) are found, but they are not constituents of normal adult tissues. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Tunafish collagen type I.
-
纯化方法
- Affinity purified
-
免疫原
- Purified collagen type I from tuna fish skin
-
亚型
- IgG
-
-
-
-
应用备注
- After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Tunafish skin,IHC(P), IFA, ELISA, RIA, IB/WB,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:500, ELISA ? 1:200 (OD ? 500)
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
浓度
- 1 mg/mL
-
缓冲液
- phosphate buffered solution, no BSA and preservative added!
-
储存液
- Without preservative
-
储存条件
- -20 °C
-
-
- Collagen Type I (COL1) (Collagen, Type I (COL1))
-
别名
- Collagen Type I
抗原
-