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- 抗原
- Crotonaldehyde (CRA)
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- HRP
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应用范围
- Western Blotting (WB), ELISA
- 特异性
- Specific for Crotonaldehyde modified proteins. Does not cross-react with Acrolein, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
- 纯化方法
- Protein G Purified
- 免疫原
- Crotonaldehyde modified Keyhole Limpet Hemocyanin (KLH)
- 克隆位点
- 2A8-1
- 亚型
- IgG1
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anti-Crotonaldehyde (CRA) antibody
WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
anti-Crotonaldehyde (CRA) antibody (Atto 390)WB, ELISA 宿主: 小鼠 Monoclonal 2A8-1 Atto 390
anti-Crotonaldehyde (CRA) antibodyWB, ELISA 宿主: 小鼠 Monoclonal 2A8-1 unconjugated
anti-Crotonaldehyde (CRA) antibody (FITC)WB, ELISA 宿主: 小鼠 Monoclonal 2A8-1 FITC
anti-Crotonaldehyde (CRA) antibody (Biotin)WB, ELISA 宿主: 小鼠 Monoclonal 2A8-1 Biotin
anti-Crotonaldehyde (CRA) antibody (PE)WB, ELISA 宿主: 小鼠 Monoclonal 2A8-1 PE
anti-Crotonaldehyde (CRA) antibody (PerCP)WB, ELISA 宿主: 小鼠 Monoclonal 2A8-1 PerCP
anti-Crotonaldehyde (CRA) antibody (APC)WB, ELISA 宿主: 小鼠 Monoclonal 2A8-1 APC
anti-Crotonaldehyde (CRA) antibody (Atto 594)WB, ELISA 宿主: 小鼠 Monoclonal 2A8-1 Atto 594
anti-Crotonaldehyde (CRA) antibody (Atto 488)WB, ELISA 宿主: 小鼠 Monoclonal 2A8-1 Atto 488
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- 应用备注
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- WB (1:1000)
- ELISA (1:1000)
- optimal dilutions for assays should be determined by the user.
- 说明
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A 1:1000 dilution of ABIN5650478 was sufficient for detection of Crotonaldehyde-modified proteins in 1 μg of Crotonaldehyde-BSA conjugate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Conjugated antibodies should be stored at 4°C
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- 抗原
- Crotonaldehyde (CRA)
- 别名
- Crotonaldehyde
- 物质类
- Chemical
- 背景
- Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. Crotonaldehyde (CRA) is a carcinogenic, conjugated aldehyde produced endogenously via lipid peroxidation. It covalently modifies biomolecules, forming adducts with DNA and nucleophilic amino acid residues of proteins such as lysine (1).
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