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Chromogranin A 抗体

CHGA 适用: 人, 大鼠, 小鼠, 猴, Pig IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal LK2H10-PHE5-CGA414 unconjugated
产品编号 ABIN5646861
发货至: 中国
  • 抗原 See all Chromogranin A (CHGA) 抗体
    Chromogranin A (CHGA)
    适用
    • 190
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    人, 大鼠, 小鼠, 猴, Pig
    宿主
    • 100
    • 95
    • 5
    • 1
    小鼠
    克隆类型
    • 117
    • 83
    单克隆
    标记
    • 84
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    This Chromogranin A antibody is un-conjugated
    应用范围
    • 106
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    • 24
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    • 11
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    Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    纯化方法
    Purified
    纯度
    Protein G affinity chromatography
    免疫原
    Human pheochromocytoma cells (LK2H10 + PHE5) and recombinant human Chromogranin A protein (CGA414) were used as the immunogen for this antibody.
    克隆位点
    LK2H10-PHE5-CGA414
    亚型
    IgG1
    Top Product
    Discover our top product CHGA Primary Antibody
  • 应用备注
    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

    限制
    仅限研究用
  • 浓度
    0.2 mg/mL
    缓冲液
    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store the Chromogranin A antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原
    Chromogranin A (CHGA)
    别名
    Chromogranin A (CHGA 产品)
    别名
    CGA antibody, CG-ALPHA antibody, FSHA antibody, GPHA1 antibody, GPHa antibody, HCG antibody, LHA antibody, TSHA antibody, ChrA antibody, fj05f09 antibody, si:dkey-177p2.2 antibody, wu:fj05f09 antibody, zgc:101749 antibody, zgc:56075 antibody, CHGA antibody, cgA antibody, chga antibody, chromogranin A antibody, glycoprotein hormones, alpha polypeptide antibody, uncharacterized CHGA antibody, chromogranin A S homeolog antibody, CHGA antibody, CGA antibody, Chga antibody, chga antibody, chga.S antibody
    背景
    Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.
    基因ID
    1113
    途径
    Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling
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