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PCNA 抗体

This anti-PCNA antibody is a 小鼠 单克隆 antibody detecting PCNA in WB, FACS, IHC (p), IP, ICC 和 IHC (fro). Suitable for 人, 小鼠, 大鼠, 小鸡, Drosophila melanogaster 和 Non-Human Primate.
产品编号 ABIN5633264
发货至: 中国

Quick Overview for PCNA 抗体 (ABIN5633264)

抗原

See all PCNA 抗体
PCNA (Proliferating Cell Nuclear Antigen (PCNA))

适用

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人, 小鼠, 大鼠, 小鸡, Drosophila melanogaster, Non-Human Primate

宿主

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小鼠

克隆类型

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单克隆

标记

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This PCNA antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

PC10
  • 原理

    Anti-PCNA Purified

    特异性

    The mouse monoclonal antibody PC10 (also known as 3F81) recognizes PCNA, a 36 kDa conserved nuclear protein serving as a cofactor for DNA synthesis.

    交叉反应 (详细)

    Human, Non-Human Primates, Mouse, Rat, Chicken, Drosophila

    纯化方法

    Purified by protein-A affinity chromatography.

    纯度

    > 95 % (by SDS-PAGE)

    免疫原

    recombinant rat PCNA

    亚型

    IgG2a kappa
  • 应用备注

    Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Store at 2-8°C. Do not freeze.
  • 抗原

    PCNA (Proliferating Cell Nuclear Antigen (PCNA))

    别名

    PCNA

    背景

    Proliferating cell nuclear antigen,PCNA (proliferating cell nuclear antigen) is the DNA polymerase delta auxiliary protein acting in homotrimeric form to increase the processivity of leading strand synthesis during DNA replication. PCNA is expressed in the nucleus of all proliferating cells. In response to DNA damage, it is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. PCNA is a useful marker of DNA synthesis, as its form not involved in DNA synthesis degradates in histological preparations in the presence of organic solvents.,ATLD2

    基因ID

    5111

    UniProt

    P12004

    途径

    Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy
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