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- 抗原
- Superoxide Dismutase
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适用
- Cow
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- HRP
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应用范围
- Western Blotting (WB), ELISA
- 交叉反应 （详细）
- Cross reactivity against Superoxide Dismutase from other tissues and species may occur but have not been specifically determined.
- 纯化方法
- Superoxide Dismutase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Superoxide Dismutase [Bovine erythrocytes].
- 免疫原
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Immunogen: Superoxide Dismutase [Bovine erythrocytes]
Immunogen Type: Native Protein
- 亚型
- IgG
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anti-Superoxide Dismutase antibody
适用: Cow, 人, 小鼠, 大鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Superoxide Dismutase (AA 113-129) antibody适用: 人, 小鼠, 大鼠 WB, IHC, IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Application Note: Anti-Superoxide Dismutase has been assayed against 1.0 μg of Superoxide Dismutase [Bovine erythrocytes] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:2,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500- 1:2,000
ELISA Dilution: 1:5,000 - 1:20,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Superoxide Dismutase
- 别名
- GTP-binding nuclear protein Ran antibody, superoxide dismutase 1 antibody, LOC786258 antibody, SOD1 antibody
- 背景
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Synonyms: ALS 1 antibody, ALS antibody, ALS1 antibody, Amyotrophic lateral sclerosis 1 adult antibody, Amyotrophic lateral sclerosis 1 antibody, Cu/Zn SOD antibody, Cu/Zn superoxide dismutase antibody, Homodimer antibody
Background: Superoxide Dismutase destroys radicals which are normally produced within the cells and which are toxic to biological systems. They are an important antioxidant defense in nearly all cells exposed to oxygen. Mutations in the Superoxide Dismutase enzyme can cause ALS, A4V, G93A, Down Syndrome, ARDS, and COPD.
Gene Name: SOD1
- 基因ID
- 281495
- UniProt
- P00442
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