Malate Dehydrogenase (MDH) 抗体 (HRP)
-
- 抗原 See all Malate Dehydrogenase (MDH) 抗体
- Malate Dehydrogenase (MDH)
-
适用
- Pig
-
宿主
-
绵羊
-
克隆类型
- 多克隆
-
标记
- HRP
-
应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
- 交叉反应 （详细）
- Cross reactivity against Malate Dehydrogenase from other sources may occur but has not been specifically determined.
- 纯化方法
- Malate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart].
- 免疫原
-
Immunogen: Malate Dehydrogenase [Pig Heart]
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product MDH Primary Antibody
-
anti-Malate Dehydrogenase (MDH) antibody
MDH 适用: Pig ELISA, WB, IP 宿主: 绵羊 Polyclonal unconjugated
-
- 应用备注
-
Immunohistochemistry Dilution: 1:500 - 1:2,500
Application Note: Anti-Malate Dehydrogenase is useful in ELISA, western blot, and immunohistochemistry. Optimal titers should be obtained by researchers.
Western Blot Dilution: 1:1,000 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:10,000 - 1:50,000
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
-
- 抗原
- Malate Dehydrogenase (MDH)
- 别名
- Malate Dehydrogenase (MDH 产品)
- 别名
- CG5889 antibody, Dmel\\CG5889 antibody, MDH antibody, Mdh antibody, anon-EST:Posey79 antibody, MALATE DEHYDROGENASE antibody, malate dehydrogenase antibody, An15g00070 antibody, AO090701000013 antibody, DDBDRAFT_0188779 antibody, DDBDRAFT_0230186 antibody, DDB_0188779 antibody, DDB_0230186 antibody, Malic enzyme b antibody, oxidoreductase, putative antibody, malate dehydrogenase antibody, NAD dependent malate dehydrogenase Mdh antibody, Men-b antibody, malate dehydrogenase antibody, MDH antibody, mdh antibody, APH_RS02635 antibody, ANI_1_12134 antibody, AOR_1_26114 antibody, PMI_RS16925 antibody, mdhA antibody, mdh1 antibody
- 背景
-
Synonyms: Malate dehydrogenase, cytoplasmic EC=1.1.1.37 Cytosolic malate dehydrogenase
Background: Malate Dehydrogenase is an enzyme that reversibly catalyzes the oxidation of malate to oxaloacetate using the reduction of NAD+ to NADH. Malate Dehydrogenase is involved in gluconeogenesis, the synthesis of glucose from smaller molecules. There are two main isoforms in eukaryotic cells. One is found in the mitochondrial matrix the other is found in the cytoplasm.
Gene Name: MDH1
- 基因ID
- 396894
- UniProt
- P11708
- 途径
- Regulation of Lipid Metabolism by PPARalpha
-
