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- 抗原
- Alpha1 -Trypsin
- 适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- HRP
- 应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- 交叉反应 （详细）
- Cross reactivity against a1-Anti-Trypsin from other sources is unknown.
- 纯化方法
- Anti-alpha-1-Anti-Trypsin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified a1-Anti-Trypsin [Human Plasma].
- 免疫原
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Immunogen: Anti-alpha 1 Anti-Trypsin Antibody was produced by repeated immunizations with native alpha1-Anti-Trypsin protein from human plasma.
Immunogen Type: Native Protein
- 亚型
- IgG
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anti-Alpha1 -Trypsin antibody
适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Alpha1 -Trypsin antibody适用: 人 ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-α-1-anti-Trypsin Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight (~47 kDa) and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:500 - 1:2,500
ELISA Dilution: 1:1,000 - 1:5,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Alpha1 -Trypsin
- 别名
- Alpha-1-Trypsin
- 背景
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Synonyms: A1AT antibody, AAT antibody, Alpha 1 antiproteinase antibody, Alpha 1 antitrypsin antibody, Alpha 1 protease inhibitor antibod
Background: Anti-alpha 1 Anti-Trypsin Antibody detects a1-Anti-Trypsin protein. a1-Anti-Trypsin is an ihibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Anti-Trypsin irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form of Antitrypsin inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Defects in SERPINA1 are the cause emphysema, liver cirrhosis and liver failure. Environmental factors, particularly cigarette smoking, greatly increase the risk of emphysema at an earlier age. Anti-alpha 1 Anti-Trypsin Antibody is ideal for investigators involved in Cell Signaling, Signal Transduction and Cancer research.
Gene Name: SERPINA1
- 基因ID
- 5265
- UniProt
- P01009
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