COL1A1 抗体 (HRP)
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- 抗原 See all COL1A1 抗体
- COL1A1 (Collagen, Type I, alpha 1 (COL1A1))
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适用
- 人, 大鼠, 小鼠, Cow
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This COL1A1 antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)
- Supplier Product No.
- 600-403-103
- Supplier
- Rockland
- 原理
- Collagen Type I Antibody Peroxidase Conjugated
- 交叉反应 (详细)
- Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
- 产品特性
- Synonyms: rabbit anti-collagen type I antibody peroxidase conjugation, HRP conjugated rabbit anti-collagen type I antibody, Collagen Of Skin Tendon And Bone, Collagen Type 1 antibody, Collagen type I alpha 1 antibody, Collagen alpha-1 (I) chain, Alpha-1 type I collagen, type 1 procollagen alpha 1
- 纯化方法
- This product has been prepared by immunoaffinity chromatography using immobilized antigens.
- 免疫原
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Immunogen: Collagen Type I from human and bovine placenta.
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product COL1A1 Primary Antibody
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- 应用备注
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Flow Cytometry Dilution: 5 μg/mL
Immunohistochemistry Dilution: 1:50 - 1:200
Application Note: Anti-COLLAGEN Type I Peroxidase Conjugated Antibody is suitable for western blot, immunoprecipitation, Flow Cytometry, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:3,000 - 1:6,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:3,000 - 1:6,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 50μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Thimerosal - 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. Restore with 0.05 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- COL1A1 (Collagen, Type I, alpha 1 (COL1A1))
- 别名
- COL1A1 (COL1A1 产品)
- 背景
- Background: COLLAGEN Type I Peroxidase Conjugated Antibody is specific for Collagen Type I. Collagen Type I (Type-I collagen) is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is also found in tendons, the endomysium of myofibrils and the organic part of bone. Anti-collagen Type I antibody is suitable for Cancer research and other general research.
- 基因ID
- 1277
- NCBI登录号
- NP_000079
- UniProt
- P02452
- 途径
- Sensory Perception of Sound, Autophagy, Growth Factor Binding
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