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D Amino Acid Oxidase 抗体 (HRP)

DAO 适用: Pig WB, ELISA, IP 宿主: 绵羊 Polyclonal HRP
产品编号 ABIN5596780
发货至: 中国
  • 抗原 See all D Amino Acid Oxidase (DAO) 抗体
    D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))
    适用
    • 36
    • 13
    • 5
    • 4
    Pig
    宿主
    • 39
    • 5
    • 3
    • 3
    绵羊
    克隆类型
    • 48
    • 2
    多克隆
    标记
    • 26
    • 7
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This D Amino Acid Oxidase antibody is conjugated to HRP
    应用范围
    • 35
    • 34
    • 10
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    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
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    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    交叉反应 (详细)
    Cross reactivity against D-Amino Acid Oxidase from other tissues and species may occur but have not been specifically determined.
    纯化方法
    D-Amino Acid Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified D-Amino Acid Oxidase [Pig Kidney].
    免疫原

    Immunogen: D-Amino Acid Oxidase [Pig Kidney]

    Immunogen Type: Native Protein

    亚型
    IgG
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  • 应用备注

    Application Note: Anti-D-Amino Acid Oxidase has been assayed against 1.0 μg of D-Amino Acid Oxidase [Pig Kidney] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:12,000 to 1:60,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:5,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:20,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    储存液
    Gentamicin sulfate
    注意事项
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))
    别名
    D-Amino Acid Oxidase (DAO 产品)
    别名
    DAAO antibody, DAMOX antibody, OXDA antibody, daao antibody, oxda antibody, damox antibody, DAO antibody, Dao1 antibody, DKFZp469N2232 antibody, AI987963 antibody, Dao-1 antibody, SC5F2A.23c antibody, wu:fb55b11 antibody, zgc:113922 antibody, ABP antibody, ABP1 antibody, DAO1 antibody, KAO antibody, MGC114783 antibody, Abp antibody, Abp1 antibody, D-amino acid oxidase antibody, D-amino-acid oxidase antibody, D-amino-acid oxidase, tandem duplicate 1 antibody, amine oxidase, copper containing 1 antibody, D-amino-acid oxidase L homeolog antibody, DAO antibody, Dao antibody, dao antibody, SCO6740 antibody, dao.1 antibody, AOC1 antibody, PTRG_02224 antibody, SJAG_02496 antibody, dao.L antibody, Aoc1 antibody
    背景

    Synonyms: D-amino-acid oxidase DAMOX DAAO DAO EC=1.4.3.3

    Background: D-Amino Acid Oxidase enzyme belongs to the FAD dependent oxidoreductase family, and acts on the CH-NH2 group of D-amino acid donors with oxygen as acceptor. It regulates the level of the neuromodulator D-serine in the brain. It has high activity towards D-DOPA and contributes to dopamine synthesis. It could act as a detoxifying agent which removes D-amino acids accumulated during aging. D-Amino Acid Oxidase acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. But does not act on acidic amino acids.

    Gene Name: DAO

    基因ID
    397134
    UniProt
    P00371
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