AKT2 抗体 (Internal Region) (APC)
-
- 抗原 See all AKT2 抗体
- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
-
抗原表位
- Internal Region
-
适用
- 人
-
宿主
- 大鼠
-
克隆类型
- 单克隆
-
标记
- This AKT2 antibody is conjugated to APC
-
应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Dot Blot (DB), Fluorescence Microscopy (FM)
- Supplier Product No.
- 200-526-j35
- Supplier
- Rockland
- 原理
- AKT2 APC Antibody
- 交叉反应 （详细）
- Anti-AKT2 antibody is directed against human AKT2. The antibody detects both unphosphorylated and phosphorylated forms of the protein.
- 产品特性
- Synonyms: rat anti-Anti-AKT2 Allophycocyanin Conjugation, APC Conjugated rat anti-Anti-AKT2, AKT 2 antibody, AKT-2, PKB antibody, PKB beta antibody, PKBBETA antibody, PRKBB antibody, Protein kinase Akt 2 antibody, Protein kinase B beta antibody, RAC-beta serine/threonine-protein kinase, RAC-PK-beta
- 免疫原
-
Immunogen: Anti-AKT2 Antibody was produced in rat by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.
Immunogen Type: Conjugated Peptide
- 克隆位点
- 11F6-B2
- 亚型
- IgG2a kappa
- Labeling Ratio
- 1-2
- Top Product
- Discover our top product AKT2 Primary Antibody
-
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 100-189) antibody
AKT2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1D9 unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 100-189) antibodyAKT2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal X1 unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 100-189) antibodyAKT2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1F8 unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 416-444) antibodyAKT2 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 93-123) antibodyAKT2 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 142-412) antibodyAKT2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (pSer474) antibodyAKT2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (C-Term) antibodyAKT2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibodyAKT2 适用: 人 WB, FACS, IP 宿主: 小鼠 Monoclonal 8D9 unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibodyAKT2 适用: 人 WB, ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 1B6 unconjugated
-
- 应用备注
-
Flow Cytometry Dilution: User Optimized
Immunohistochemistry Dilution: User Optimized
Application Note: Anti-AKT2 APC Antibody is tested for Flow Cytometry. This antibody is suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT2 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
IF Microscopy Dilution: User Optimized
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution Volume: 50μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: None - 储存液
- Without preservative
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
- 有效期
- 12 months
-
- 抗原
- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
- 别名
- AKT2 (AKT2 产品)
- 背景
- Background: AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT2 (RAT) APC conjugated Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.
- 基因ID
- 208
- NCBI登录号
- NP_001617
- UniProt
- P31751
- 途径
- PI3K-Akt Signaling, RTK signaling, AMPK Signaling, TLR signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling
-
