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Phosphothreonine 抗体

Name: Phosphothreonine 抗体
SKU: ABIN5585637
Availability: OutOfStock

WB, ELISA, IP, IHC, ICC, FACS 宿主: 兔 Monoclonal RM102 unconjugated

Western blot analysis of Lane 1: serum-starved A431 cells, Lane 2: serum-starved A431 cells treated with Calyculin A/Okadaic Acid using Phosphothreonine monoclonal antibody, clone RM102 at 1:2000 dilution. (Phosphothreonine 抗体)

Immunoprecipitation analysis of Lane 1: Calyculin A/Okadaic Acid-treated A431 cell whole lysates, Lane 2: Calyculin A/Okadaic Acid-treated A431 cell whole lysates using rabbit IgG antibody; Lane 3: Calyculin A/Okadaic Acid-treated A431 cell whole lysates using Phosphothreonine monoclonal antibody, clone RM102 at 1:500 dilution. (Phosphothreonine 抗体)

Immunocytochemistry staining of serum-starved A431 cells (A) and serum-starved A431 cells treated with Calyculin A/Okadaic Acid (B) using Phosphothreonine monoclonal antibody, clone RM102 (Red) at 1:500 dilution. (Phosphothreonine 抗体)

ICC

3 images

产品编号 ABIN5585637

发货至: 中国

Datasheet as PDF

抗原 See all Phosphothreonine products

Phosphothreonine
宿主
16

7
兔
Compatible Secondaries
克隆类型
15

8
单克隆
标记
14

2

2

2

1

1

1
This Phosphothreonine antibody is un-conjugated
应用范围
22

19

17

7

6

6

5

2

1

1

1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

原理

Recombinant rabbit monoclonal antibody raised against phosphothreonine.

特异性

This antibody reacts threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated threonine, phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides.

免疫原

Original antibody is raised against a mixture of phosphothreonine conjugated with BSA and phosphothreonine containing peptide.

克隆位点

RM102

亚型

IgG
anti-Phosphothreonine antibody
WB, ELISA, IP, ICC, IF 宿主: 兔 Polyclonal unconjugated

anti-Phosphothreonine antibody (FITC)
WB, ELISA, IP, ICC, IF 宿主: 兔 Polyclonal FITC

anti-Phosphothreonine antibody (Biotin)
WB, ELISA, IP, ICC, IF 宿主: 兔 Polyclonal Biotin

anti-Phosphothreonine antibody (Atto 488)
WB, ELISA, IP, ICC, IF 宿主: 兔 Polyclonal Atto 488

anti-Phosphothreonine antibody (Atto 594)
WB, ELISA, IP, ICC, IF 宿主: 兔 Polyclonal Atto 594

anti-Phosphothreonine antibody (Atto 390)
WB, ELISA, IP, ICC, IF 宿主: 兔 Polyclonal Atto 390

anti-Phosphothreonine antibody
WB, ELISA 宿主: 小鼠 Monoclonal 18F6 unconjugated

anti-Phosphothreonine (phosphorylated) antibody (HRP)
WB, ELISA, IP, IHC 宿主: 兔 Polyclonal HRP

anti-Phosphothreonine (pThr) antibody
WB, ELISA, IP, IHC 宿主: 兔 Polyclonal unconjugated

anti-Phosphothreonine antibody
ELISA, IP, IHC, FACS, RIA 宿主: 小鼠 Monoclonal 18F6 unconjugated

应用备注

ELISA
Flow Cytometry
Immunocytochemistry (1:100-1:500)
Immunohistochemistry (1:100-1:500)
Immunoprecipitation (1:100-1:500)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.

限制

仅限研究用
状态

Liquid

缓冲液

In PBS (50 % glycerol, 1 % BSA, 0.09 % sodium azide)

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
抗原

Phosphothreonine

Abstract

Phosphothreonine 产品

物质类

Amino Acid