(1 validation)-
- 抗原 See all CNST 抗体
- CNST (Consortin, Connexin Sorting Protein (CNST))
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CNST antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 原理
- Rabbit polyclonal antibody raised against partial recombinant Cnst.
- 序列
- MAPEERRDSE DRVSKETEDY LHSLLERCLK DAEDSLSYED IQDDDSDLLQ DLSPEEASYS LQEDLPPDES TLSLDDLAKK IEIAEAIPAE GLVSILKKRN DTVGSHPAQM QQKPAKRRVR FQEID
- 特异性
- Specific to recombinant protein GX2268. This antibody detects mC1orf71 protein.
- 交叉反应
- 小鼠
- 免疫原
- Recombinant GST fusion protein corresponding to 125 amino acids of mouse Cnst.
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anti-Consortin, Connexin Sorting Protein (CNST) antibody
CNST 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Consortin, Connexin Sorting Protein (CNST) antibodyCNST 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Consortin, Connexin Sorting Protein (CNST) (AA 1189-1435) antibodyCNST 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Consortin, Connexin Sorting Protein (CNST) antibodyCNST 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western Blot (1:1000)
The optimal working dilution should be determined by the end user. - 限制
- 仅限研究用
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- by
- Christoffels Lab, Heart Failure Research Center, Academisch Medisch Centrum Amsterdam
- No.
- #102674
- 日期
- 2018.05.14
- 抗原
- CNST
- Lot Number
- BIAD001
- Method validated
- Western Blotting
- Positive Control
- Whole E13.5 wildtype mouse embryo protein lysate
- Negative Control
- Whole E13.5 mouse embryo protein lysates homozygous for a null Cnst allele. This mouse line was generated using CRISPR/Cas9.
- Notes
Passed. ABIN5575708 specifically recognizes Cnst in whole embryo lysates.
- Primary Antibody
- ABIN5575708
- Secondary Antibody
- anti-rabbit HRP-conjugated antibody (GE Healthcare, NA934V, lot 9804671)
- Full Protocol
- Isolate E13.5 mouse embryos in cold PBS and snap freeze with liquid nitrogen.
- Lyse each individual embryo in 300µl of cold RIPA buffer.
- Determine total protein content of the lysates using Pierce™ BCA Protein Assay Kit (Thermo Scientific, 23227, lot NA168207).
- Denature 5µg of total protein for 5min at 95°C in 20µl 2x Laemmli SDS buffer and subsequently separate them on a 10% denaturing acrylamide gel in a BIO-RADMini-PROTEAN 3 chamber for 1h at 180V.
- Transfer proteins onto PVDF membrane (Immobilon-P, Millipore) with a Western blotting system for 45min at 25V.
- Block the membrane with 5% Milk in TBST as blocking buffer for 1-2h at RT.
- Incubation with primary
- rabbit anti-Consortin antibody (antibodies-online, ABIN5575708, lot BIAD001) diluted 1:2000 in blocking buffer 5% milk ON at 4°C.
- mouse anti-GAPDH antibody (Santa Cruz, sc-365062, lot I0916) diluted 1:5000 in blocking buffer ON at 4°C.
- Wash membrane 5x for 15min with TBST.
- Incubation with secondary
- anti-rabbit HRP-conjugated antibody (GE Healthcare, NA934V, lot 9804671) diluted 1:2000 blocking buffer for 1-2h at RT.
- anti-mouse HRP-conjugated antibody (GE Healthcare, NA931V, lot 9800151) diluted 1:2000 in blocking buffer for 1-2h at RT.
- Wash membrane 5x for 15min with TBST.
- Reveal protein bands using ECL detection kit (GE Healthcare) on an ImageQuant LAS 4000 phosphoimaging unit (GE Healthcare).
- Experimental Notes
The Consortin antibody ABIN5575708 reveals a protein of the expected molecular weight of antigen in lysates of whole E13.5 embryos. The protein bands is only visible in the positive but not the negative controls.
生效 #102674 (Western Blotting)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Western blot detection of consortin whole E13.5 mouse embryo lysates using ABIN5575708. Each lane represents one whole E13.5 embryo lysate of each specified genotype, with CNST at about 75kDa and control GAPDH at about 35kDa.]()
Western blot detection of consortin whole E13.5 mouse embryo lysates using ABIN5575708. Each lane represents one whole E13.5 embryo lysate of each specified genotype, with CNST at about 75kDa and control GAPDH at about 35kDa.
Full Methods -
- 状态
- Liquid
- 缓冲液
- In PBS (50 % glycerol, 0.02 % sodium azide)
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
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Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
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- 抗原
- CNST (Consortin, Connexin Sorting Protein (CNST))
- 别名
- 9630058J23Rik (CNST 产品)
- 别名
- C1orf71 antibody, PPP1R64 antibody, 9630058J23Rik antibody, consortin, connexin sorting protein antibody, CNST antibody, Cnst antibody
- 背景
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Full Gene Name: RIKEN cDNA 9630058J23 gene
Synonyms: MGC31547 - 基因ID
- 226744
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