Peptide ELISA: 1/32000. Western Blot: 0.5 - 1 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Roundabout proteins 1-3, known as Robo1-3, are repulsive axon guidance receptors that bind to Slit, a repellent secreted by midline glia during development. Robo3 (also known as Rig1) is important for normal axon navigation at the ventral midline of the neural tube during development and is specifically expressed by commissural axons in rodents. Loss-of-function studies in mice show that Robo3 is required to allow commissural axons to enter the floor plate and cross to the contralateral side of the spinal cord. Some isoforms of Robo3 exist.Synonyms: ROBO-3, Roundabout homolog 3, Roundabout-like protein 3