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VEGFA 抗体 (AA 288-299)

The 山羊 多克隆 anti-VEGFA antibody (ABIN5539606) specifically detects VEGFA in WB, IHC 和 ELISA. The antibody is reactive with 人 samples.
产品编号 ABIN5539606
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Quick Overview for VEGFA 抗体 (AA 288-299) (ABIN5539606)

抗原

See all VEGFA 抗体
VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

适用

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人

宿主

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山羊

克隆类型

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多克隆

标记

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This VEGFA antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA

质量等级

Verified
  • 抗原表位

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    AA 288-299

    原理

    VEGFA (aa288-299)

    序列

    RIKPHQGQHI GE

    特异性

    This antibody is expected to recognize all reported isoforms.

    交叉反应

    犬, 人, Pig

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Liver and Small Intestine. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 45 kDa band observed in lysates of cell line HEK293 (calculated MW of 45.5 kDa according to NP_001020537.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat

    Peptide ELISA: antibody detection limit dilution 1:128000.

    说明

    Immunofluorescence: Testing has shown that this antibody stains mitochondria in Mouse NIH3T3 cells at 10μg/ml.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

    别名

    VEGFA

    背景

    VEGFA, vascular endothelial growth factor A, MGC70609, MVCD1, VEGF, VPF, OTTHUMP00000016487, OTTHUMP00000016488, OTTHUMP00000165985, OTTHUMP00000165986, OTTHUMP00000165987, OTTHUMP00000224107, OTTHUMP00000224108, OTTHUMP00000224109, OTTHUMP00000224153, OT

    基因ID

    7422

    NCBI登录号

    NP_001020537, NP_003367, NP_001020538, NP_001020539

    途径

    RTK signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
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