This antibody is purified through a protein A column, followed by peptide affinity purification.
免疫原
This CENPH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136-162 amino acids from the Central region of human CENPH.
CENPH
适用: 人
WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro)
宿主: 兔
Polyclonal
unconjugated
应用备注
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
限制
仅限研究用
状态
Liquid
浓度
0.5 mg/mL
缓冲液
Supplied in PBS with 0.09 % (W/V) sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1700021I11Rik antibody, 2410018A12Rik antibody, 2610042E16Rik antibody, 2810046K12Rik antibody, AU044255 antibody, CENP-H antibody, ENP antibody, si:dkey-186m8.3 antibody, cenp-h antibody, centromere protein H antibody, centromere protein H pseudogene antibody, CENPH antibody, Cenph antibody, cenph antibody, LOC100356112 antibody, LOC101788801 antibody
背景
Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.