Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
纯化方法
This antibody is purified through a protein A column, followed by peptide affinity purification.
免疫原
This CYP2B6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-263 amino acids from the Central region of human CYP2B6.
CYP2B6
适用: 人, 小鼠
WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro)
宿主: 兔
Polyclonal
unconjugated
应用备注
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
限制
仅限研究用
状态
Liquid
浓度
0.36 mg/mL
缓冲液
Supplied in PBS with 0.09 % (W/V) sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
抗原
CYP2B6
(Cytochrome P450, Family 2, Subfamily B, Polypeptide 6 (CYP2B6))
CPB6 antibody, CYP2B antibody, CYP2B7 antibody, CYP2B7P antibody, CYPIIB6 antibody, EFVM antibody, IIB1 antibody, P450 antibody, CPF3 antibody, CYP4F antibody, LTB4H antibody, CYP2B30 antibody, CYP2B11 antibody, CYPIIB11 antibody, 21-OH antibody, 21OH antibody, 21OHA antibody, 21OHB antibody, CYP21OH-A antibody, Cyp21 antibody, Cyp21-ps1 antibody, Cyp21B antibody, Cyp21a2-ps antibody, Cyp21a2ps antibody, Oh21-1 antibody, Oh21-2 antibody, CYP2B6 antibody, cytochrome P450 family 2 subfamily B member 6 antibody, cytochrome P450 family 4 subfamily F member 3 antibody, cytochrome P450, family 2, subfamily B, polypeptide 6 antibody, cytochrome P450 family 2 subfamily B member 6 L homeolog antibody, cytochrome P450 2B11 antibody, cytochrome P450, family 21, subfamily a, polypeptide 1 antibody, cytochrome P450 subfamily 2B antibody, cytochrome P450 2B11-like antibody, CYP2B6 antibody, CYP4F3 antibody, cyp2b6.L antibody, Cyp21a1 antibody, LOC100068603 antibody
背景
This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described, however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q.