RAF1 抗体 (AA 364-451)
(1 reference)Quick Overview for RAF1 抗体 (AA 364-451) (ABIN5518949)
抗原
See all RAF1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 364-451
-
原理
- Anti-Raf1 Antibody Picoband®
-
交叉反应 （详细）
- No cross-reactivity with other proteins.
-
产品特性
- Anti-Raf1 Antibody Picoband® (ABIN5518949). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E. coli-derived human Raf1 recombinant protein (Position: Y364-Q451). Human Raf1 shares 98.9% amino acid (aa) sequence identity with both mouse and rat Raf1.
-
亚型
- IgG
-
-
anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 31-130) antibody
RAF1 适用: 人 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 190-350) antibodyRAF1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 1-130) antibodyRAF1 适用: 人 WB, ELISA, PLA, RNAi 宿主: 小鼠 Monoclonal 1H4 unconjugated
anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibodyRAF1 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 4G4 unconjugated
anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (pSer621) antibodyRAF1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (pSer259) antibodyRAF1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 1-648) antibodyRAF1 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibodyRAF1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (pSer338) antibodyRAF1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (pSer621) antibodyRAF1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Li P, Wood K, Mamon H, Haser W, Roberts T (February 1991). "Raf-1: a kinase currently without a cause but not lacking in effects". Cell. 64 (3): 479-82. 2. Rapp UR, Goldsborough MD, Mark GE, Bonner TI, Groffen J, Reynolds FH, Stephenson JR (July 1983). "Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus". Proc. Natl. Acad. Sci. U.S.A. 80 (14): 4218-22. -
说明
-
We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
-
-
-
: "The role of Dectin-1/Raf-1 signal cascade in innate immune of human corneal epithelial cells against Aspergillus fumigatus infection." in: International journal of ophthalmology, Vol. 9, Issue 10, pp. 1371-1375, (2016) (PubMed).
-
: "The role of Dectin-1/Raf-1 signal cascade in innate immune of human corneal epithelial cells against Aspergillus fumigatus infection." in: International journal of ophthalmology, Vol. 9, Issue 10, pp. 1371-1375, (2016) (PubMed).
-
- RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
-
别名
- RAF1
-
背景
-
Synonyms: RAF proto-oncogene serine/threonine-protein kinase,2.7.11.1,Proto-oncogene c-RAF,cRaf,Raf-1,RAF1,RAF,
Tissue Specificity: In skeletal muscle, isoform 1 is more abundant than isoform 2. .
Background: RAF proto-oncogene serine/threonine-protein kinase, also known as proto-oncogene c-RAF or simply c-Raf or even Raf-1, is an enzyme that in humans is encoded by the RAF1 gene. This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinasekinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds ly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
-
分子量
- 73 kDa
-
基因ID
- 5894
-
UniProt
- P04049
-
途径
- MAPK Pathway, RTK signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling
抗原
-
