SPARC 抗体 (AA 107-303)
Quick Overview for SPARC 抗体 (AA 107-303) (ABIN5518870)
抗原
See all SPARC 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 107-303
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原理
- Anti-SPARC Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-SPARC Antibody Picoband® (ABIN5518870). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human SPARC recombinant protein (Position: E107-I303). Human SPARC shares 97% amino acid (aa) sequence identity with both mouse and rat SPARC.
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亚型
- IgG
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anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 101-200) antibody
SPARC 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 1-303) antibodySPARC 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1B2 unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 224-251) antibodySPARC 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) antibodySPARC 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 284-303) antibodySPARC 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 224-251), (C-Term) antibodySPARC 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB19869 unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) antibodySPARC 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-302) antibodySPARC 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 284-303), (C-Term) antibodySPARC 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 1-303) antibodySPARC 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Gilmour, D. T., Lyon, G. J., Carlton, M. B. L., Sanes, J. R., Cunningham, J. M., Anderson, J. R., Hogan, B. L. M., Evans, M. J., Colledge, W. H.Mice deficient for the secreted glycoprotein SPARC/osteonectin/BM40 develop normally but show severe age-onset cataract formation and disruption of the lens.EMBO J. 17: 1860-1870, 1998. 2. Goldblum, S. E., Ding, X., Funk, S. E., Sage, E. H.SPARC (secreted protein acidic and rich in cysteine) regulates endothelial cell shape and barrier function.Proc. Nat. Acad. Sci. 91: 3448-3452, 1994. 3. Minn, A. J., Gupta, G. P., Siegel, P. M., Bos, P. D., Shu, W., Giri, D. D., Viale, A., Olshen, A. B., Gerald, W. L., Massague, J.Genes that mediate breast cancer metastasis to lung.Nature 436: 518-524, 2005. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))
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别名
- SPARC
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背景
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Synonyms: SPARC,Basement-membrane protein 40,BM-40,Osteonectin,ON,Secreted protein acidic and rich in cysteine,SPARC,ON,
Tissue Specificity: Expressed in liver, lung and heart right ventricle.
Background: SPARC, secreted protein acidic and rich in cysteine, also known as Osteonectin is a protein that in humans is encoded by the SPARC gene. The human SPARC gene is 26.5 kb long, and contains 10 exons and 9 introns and is located on chromosome 5q31-q33. SPARC is an acidic, cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus (domain I), 2) a cysteine- rich (domain II), 3) a hydrophilic region (domain III) and 4) an EF hand motif at the carboxy terminus region (domain IV). Furthermore, SPARC is a glycoprotein in the bone that binds sodium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. SPARC also shows affinity for collagen in addition to bone mineral calcium. A correlation between SPARC over expression and ampullary cancers and chronic pancreatitis has been found.
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分子量
- 35 kDa
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基因ID
- 6678
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UniProt
- P09486
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途径
- Autophagy
抗原
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