Nemo-Like Kinase 抗体 (N-Term)
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- 抗原 See all Nemo-Like Kinase (NLK) 抗体
- Nemo-Like Kinase (NLK)
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抗原表位
- N-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Nemo-Like Kinase antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 序列
- SSAAAAAAAA AAAAAMLNPG QQQPYFPSPA PGQAPGPAAA APAQVQAAAA
- 产品特性
- This is a rabbit polyclonal antibody against NLK. It was validated on Western Blot.
- 纯化方法
- Affinity purified
- 免疫原
- The immunogen is a synthetic peptide directed towards the N-terminal region of Human NLK
- Top Product
- Discover our top product NLK Primary Antibody
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anti-Nemo-Like Kinase (NLK) (AA 416-515) antibody
NLK 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 2B11 unconjugated
anti-Nemo-Like Kinase (NLK) antibodyNLK 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Nemo-Like Kinase (NLK) antibodyNLK 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Nemo-Like Kinase (NLK) (AA 278-527) antibodyNLK 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nemo-Like Kinase (NLK) (AA 278-527) antibodyNLK 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nemo-Like Kinase (NLK) (AA 1-515) antibodyNLK 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Nemo-Like Kinase (NLK) (N-Term) antibodyNLK 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nemo-Like Kinase (NLK) (AA 275-300) antibodyNLK 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB16621 unconjugated
anti-Nemo-Like Kinase (NLK) (AA 408-436), (C-Term) antibodyNLK 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB34651 unconjugated
anti-Nemo-Like Kinase (NLK) (Thr286) antibodyNLK 适用: 人 WB, FACS, IF/ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- 抗原
- Nemo-Like Kinase (NLK)
- 别名
- NLK (NLK 产品)
- 别名
- Amf antibody, Gpi1 antibody, AI194375 antibody, nlk antibody, nlkb antibody, zgc:111875 antibody, Xnlk antibody, nlk1 antibody, nlk2 antibody, NLK antibody, nemo-like antibody, RGD1561602 antibody, glucose-6-phosphate isomerase antibody, nemo like kinase antibody, nemo-like kinase, type 1 antibody, nemo-like kinase L homeolog antibody, Nemo-Like kinase antibody, nemo-like kinase antibody, Gpi antibody, NLK antibody, Nlk antibody, nlk1 antibody, nlk.L antibody, nlk antibody, nemo-like antibody
- 背景
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NLK is a serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. It is a positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK- SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. It binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. It is a negative regulator of the Notch signaling pathway. It binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1.
Alias Symbols: NLK, LAK1 {ECO:0000312|EMBL:AAD56013.1
Protein Interaction Partner: KRTAP5-6, KRTAP10-3, KRTAP4-2, QRICH1, ZHX3, KRTAP5-9, GRN, TP53, MDM2, STAT5A, LEF1, CNOT2, SMAD4, KPNB1, BACH1, TNKS1BP1, FAM222A, C2orf44, CEP97, RNF219, FAM222B, CNOT11, NLK, PASK, SHANK2, UBAP2L, CCP110, TRPS1, TLE3, SKI, RANGAP1, RANBP2, PKM, CNOT3,
Protein Size: 527 - 基因ID
- 51701
- NCBI登录号
- NP_057315
- UniProt
- Q9UBE8
- 途径
- Ubiquitin Proteasome Pathway
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