Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
原理
Rabbit polyclonal antibody raised against full length recombinant MARCKS.
特异性
The MARCKS protein runs at about 80KDa on SDS-PAGE gels, although the real molecular weight is much lower, at about 31.5KDa. The reason for this is probably because the protein is extremely rich in acidic residues. Such proteins appear to bind less SDS than the average protein, and so migrate in PAGE more slowly than the normal protein.
免疫原
Recombinant protein corresponding to full length MARCKS.
Immunocytochemistry (1:5000) Immunofluorescence (1:500-1:1000) Western Blot (1:20000) The optimal working dilution should be determined by the end user.
限制
仅限研究用
状态
Liquid
缓冲液
In antiserum
储存条件
4 °C,-20 °C
储存方法
Store at 4°C for short term. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
抗原
MARCKS
(Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))