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7-Ketocholesterol (7-KC) 抗体 (APC)

ELISA, ICC, IF, WB 宿主: 小鼠 Monoclonal 3F7 APC
产品编号 ABIN5067335
发货至: 中国
  • 抗原
    7-Ketocholesterol (7-KC)
    宿主
    • 22
    小鼠
    克隆类型
    • 22
    单克隆
    标记
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    APC
    应用范围
    • 21
    • 21
    • 21
    • 21
    • 1
    • 1
    ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
    特异性
    Specific for 7-ketocholesterol modified proteins.
    纯化方法
    Protein G Purified
    免疫原
    Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH).
    克隆位点
    3F7
    亚型
    IgG3
  • 应用备注
    • WB (1:1000)
    • ICC/IF (1:50)
    • ELISA (1:1000)
    • optimal dilutions for assays should be determined by the user.
    说明

    A 1:1000 dilution of ABIN5067335 was sufficient for detection of 7-Ketocholesterol in 2 μg of 7-ketocholesterol conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Conjugated antibodies should be stored at 4°C
  • 抗原
    7-Ketocholesterol (7-KC)
    别名
    7-Ketocholesterol
    物质类
    Hormone
    背景
    Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. 7-ketocholesterol (7-KC) is a biomarker of lipoprotein cholesterol oxidation.
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