ERK1 抗体 (pThr202, pTyr204)
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Quick Overview for ERK1 抗体 (pThr202, pTyr204) (ABIN498784)
抗原
See all ERK1 (MAPK3) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr202, pTyr204
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特异性
- This antibody detects endogenous levels of p-ERK1/2 protein only when phosphorylated at Thr202/Y204.
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity chromatography
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纯度
- > 95 % by SDS-PAGE
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anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 101-200) antibody
MAPK3 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibodyMAPK3 适用: 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 1-100) antibodyMAPK3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (pTyr204) antibodyMAPK3 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibodyMAPK3 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 1E5 unconjugated
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (Atto 390)MAPK3 适用: 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal Atto 390
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (Atto 488)MAPK3 适用: 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal Atto 488
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (Atto 594)MAPK3 适用: 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal Atto 594
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (APC)MAPK3 适用: 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal APC
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (HRP)MAPK3 适用: 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal HRP
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应用备注
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Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- PBS, pH ~7.2, 0.05 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))
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别名
- MAPK3 / ERK1
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背景
- The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK 1 and Threonine 185 and Tyrosine 187 residues of ERK 2 is required for full enzymatic activation. The structural consequences of dual phosphorylation in ERK 2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.Synonyms: ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 3, PRKM3, p44-ERK1, p44-MAPK
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分子量
- approx. 42,44 kDa
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基因ID
- 5595
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NCBI登录号
- NP_002737
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UniProt
- P27361
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途径
- MAPK Pathway, RTK signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins
抗原
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