IRS1 抗体 (pSer307)
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Quick Overview for IRS1 抗体 (pSer307) (ABIN498715)
抗原
See all IRS1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer307
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特异性
- This antibody detects endogenous levels of IRS-1 protein only when phosphorylated at Ser307.
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity chromatography
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纯度
- > 95 % by SDS-PAGE
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anti-Insulin Receptor Substrate 1 (IRS1) (pSer312) antibody
IRS1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin Receptor Substrate 1 (IRS1) (pSer636) antibodyIRS1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin Receptor Substrate 1 (IRS1) (pSer639) antibodyIRS1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin Receptor Substrate 1 (IRS1) antibodyIRS1 适用: 人, 小鼠, 大鼠, Pig WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Insulin Receptor Substrate 1 (IRS1) (AA 1041-1242) antibodyIRS1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin Receptor Substrate 1 (IRS1) (pSer307) antibodyIRS1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin Receptor Substrate 1 (IRS1) (AA 106-122), (N-Term) antibodyIRS1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Insulin Receptor Substrate 1 (IRS1) (Ser312) antibodyIRS1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Insulin Receptor Substrate 1 (IRS1) (pSer636) antibodyIRS1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Insulin Receptor Substrate 1 (IRS1) (AA 1041-1242) antibodyIRS1 适用: 人 WB, IHC, IF, FACS, ICC 宿主: 小鼠 Monoclonal 10I3 unconjugated
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应用备注
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Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- IRS1 (Insulin Receptor Substrate 1 (IRS1))
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别名
- IRS1
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背景
- Insulin receptor substrate-1 (IRS-1) is a 170-185 kDa substrate of the insulin receptor that undergoes phosphorylation in response to insulin, IGF-1 and IL-4. Tyrosine (Tyr) phosphorylation of IRS-1 mediates insulin-stimulated responses, while Serine (Ser)/Threonine (Thr) phosphorylation of IRS-1 can either enhance or negate insulin effects. Tyrosines 465, 612, 632, 662, 941 and 989 of IRS-1 resemble YXXM motifs that upon phosphorylation are predicted to bind SH2 domains in the p85 regulatory subunit of PI3K, resulting in activation of p110 catalytic subunit. SHP-2 binding to IRS-1 can occur upon phosphorylation at Tyr 1179 and Tyr 1229. GRB2 binding can occur upon phsophorylation at Tyr 896. Rodent Ser 99 and Thr 502 of IRS-1 are casein kinase II-dependent phosphorylation sites. There is an increase in Ser 636 phosphorylation of IRS-1 in primary skeletal muscle cells from patients with type 2 diabetes.Synonyms: IRS-1, Insulin receptor substrate 1
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分子量
- approx. 132, 180 kDa
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基因ID
- 3667
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NCBI登录号
- NP_005535
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UniProt
- P35568
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途径
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process
抗原
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