Connexin 43/GJA1 抗体
Quick Overview for Connexin 43/GJA1 抗体 (ABIN498197)
抗原
See all Connexin 43/GJA1 (GJA1) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of Connexin 43 protein.
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Affinity-Chromatography using epitope-specific immunogen
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anti-Gap Junction Protein, alpha 1, 43kDa (GJA1) (C-Term) antibody
GJA1 适用: 人, 大鼠, 小鼠, 犬, Cow, 猴, 兔, 小鸡, 豚鼠, Pig, 马, 绵羊, Hamster, 山羊, 驴, Avian, Cat WB, IF, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated
anti-Gap Junction Protein, alpha 1, 43kDa (GJA1) (pSer368) antibodyGJA1 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Gap Junction Protein, alpha 1, 43kDa (GJA1) (AA 107-138) antibodyGJA1 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Gap Junction Protein, alpha 1, 43kDa (GJA1) (AA 351-367) antibodyGJA1 适用: 人, 大鼠, 小鼠 WB, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Gap Junction Protein, alpha 1, 43kDa (GJA1) (AA 211-260) antibodyGJA1 适用: 人, 大鼠, 小鼠, 犬 WB, ELISA, IHC (p), IF (p), FACS, IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Gap Junction Protein, alpha 1, 43kDa (GJA1) (AA 244-382) antibodyGJA1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Gap Junction Protein, alpha 1, 43kDa (GJA1) (AA 233-382) antibodyGJA1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Gap Junction Protein, alpha 1, 43kDa (GJA1) (C-Term) antibodyGJA1 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Gap Junction Protein, alpha 1, 43kDa (GJA1) (AA 233-382) antibodyGJA1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Gap Junction Protein, alpha 1, 43kDa (GJA1) (N-Term) antibodyGJA1 适用: 人, 大鼠, 小鼠, 犬, Cow, 兔, 豚鼠, 斑马鱼, 马, 绵羊 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))
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别名
- GJA1 / Cx43
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背景
- The connexins are a group of gap junction proteins which form a hexamer to compose a connexon. Clusters of connexons form a gap junction through which low molecular weight proteins may diffuse from cell to cell. Several mammalian cells with malignant phenotypes exhibit decreased connexin expression and gap junction communication. In Src transformed cells, there is a decrease in gap junctional communication, which appears to be associated with tyrosine phosphorylation of connexin 43. Activated c-Src phosphorylates the C-terminal tail of connexin 43 on Tyr 265, resulting in a stable interaction between both proteins, which leads to inhibition of gap junctional communication. In addition to tyrosine phosphorylation, connexin 43 has also been shown to be phosphorylated on serine in the ab-sence of Src kinases and on both serine and tyrosine in cells expressing Src kinases, such as c-Src and/or pp60v-Src. In human vascular endothelial cells, connexin 43 is posttranslationally modified during mitosis. Mitosis-specific phosphorylation of connexin 43 correlates with the transient loss of gap junction intercellular communication and redistribution of connexin 43.Synonyms: Connexin-43, GJAL, Gap junction 43 kDa heart protein, Gap junction alpha-1 protein
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分子量
- approx. 43 kDa
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基因ID
- 2697
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NCBI登录号
- NP_000156
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UniProt
- P17302
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途径
- MAPK Pathway, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization
抗原
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