GSK3 beta 抗体
Quick Overview for GSK3 beta 抗体 (ABIN4953256)
抗原
See all GSK3 beta (GSK3b) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity
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免疫原
- A recombinant partial protein was used as the immunogen for this GSK3B antibody.
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亚型
- IgG2a
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anti-Glycogen Synthase Kinase 3 beta (GSK3b) (AA 4-34), (N-Term) antibody
GSK3b 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB03816 unconjugated
anti-Glycogen Synthase Kinase 3 beta (GSK3b) antibodyGSK3b 适用: 人, 小鼠 WB, IF, IP 宿主: 小鼠 Monoclonal 2E6-D6-C12 unconjugated
anti-Glycogen Synthase Kinase 3 beta (GSK3b) (N-Term) antibodyGSK3b 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Glycogen Synthase Kinase 3 beta (GSK3b) (AA 30-59) antibodyGSK3b 适用: 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Glycogen Synthase Kinase 3 beta (GSK3b) (AA 4-34) antibodyGSK3b 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Glycogen Synthase Kinase 3 beta (GSK3b) (AA 2-33) antibodyGSK3b 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal RB56641 unconjugated
anti-Glycogen Synthase Kinase 3 beta (GSK3b) (AA 362-395) antibodyGSK3b 适用: 大鼠 WB 宿主: 兔 Polyclonal RB56577 unconjugated
anti-Glycogen Synthase Kinase 3 beta (GSK3b) (AA 2-33), (pSer9) antibodyGSK3b 适用: 人 WB 宿主: 兔 Polyclonal RB56641 unconjugated
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应用备注
- The stated application concentrations are suggested starting points. Titration of the GSK3B antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,ICC/IF: 1:200,Immunoprecipitation: suitable
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限制
- 仅限研究用
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缓冲液
- Antibody in PBS with 50 % Glycerol and 0.03 % ProClin 300
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储存条件
- -20 °C
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储存方法
- Store the GSK3B antibody at -20°C.
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- GSK3 beta (GSK3b) (Glycogen Synthase Kinase 3 beta (GSK3b))
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别名
- GSK3 beta / GSK3B
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背景
- Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase. In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. [UniProt]
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UniProt
- P49841
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途径
- WNT signaling, Hedgehog Signaling, Fc-epsilon Receptor Signaling Pathway, Cellular Glucan Metabolic Process, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Autophagy, BCR Signaling, Warburg Effect
抗原
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