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- 抗原
- MGA
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适用
- 人
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- 非结合性
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应用范围
- Western Blotting (WB)
- 纯化方法
- Antigen affinity
- 免疫原
- Amino acids QKEAEAFAYYRRTHTANERRRRGEMRDLFEKLKITLGLLH of human MGA were used as the immunogen for the MGA antibody.
- 亚型
- IgG
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anti-MGA antibody
适用: 人 IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-MGA (AA 2376-2415), (C-Term) antibody适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-MGA (C-Term) antibody适用: 人, Pig, 兔, 大鼠, Cow, 犬, 马, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal dilution of the MGA antibody should be determined by the researcher.\. Western blot: 0.1-0.5 μg/mL
- 限制
- 仅限研究用
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- 缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
- 储存条件
- -20 °C
- 储存方法
- After reconstitution, the MGA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
-
- 抗原
- MGA
- 背景
- Mga is a DNA-binding protein that activates the expression of several important virulence genes in Streptococcus pyogenes (group A Streptococcus, GAS) in response to changing environmental conditions. It had been found that the mouse transcription factor Max interacted with Mga. Coimmunoprecipitation analysis confirmed that Max and Mga interacted in transfected HEK293 cells. EMSA revealed that Mga required Max for binding to the E-box sequence CACGTG. The isolated T-box of Mga bound the brachyury T-box-binding site in the absence of Max. Mga repressed transcription of a reporter driven from a T-box-binding site, but coexpression of Mga with Max caused transcriptional activation from the T-box-binding site. Expression of Mga with Max, but not Mga alone, activated transcription from a reporter containing the E-box site.
- UniProt
- Q8IWI9
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