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- 抗原 See all B3GNT8 抗体
- B3GNT8 (UDP-GlcNAc:betaGal beta-1,3-N-Acetylglucosaminyltransferase 8 (B3GNT8))
- 适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This B3GNT8 antibody is un-conjugated
- 应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
- 纯化方法
- Antigen affinity
- 免疫原
- Amino acids ADRTADHCAFRNLLLVRPLGPQASIRLWKQLQDPRLQC of human B3GNT8 were used as the immunogen for the B3GNT8 antibody.
- 亚型
- IgG
- Top Product
- Discover our top product B3GNT8 Primary Antibody
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anti-UDP-GlcNAc:betaGal beta-1,3-N-Acetylglucosaminyltransferase 8 (B3GNT8) (AA 360-397), (C-Term) antibody
B3GNT8 适用: 人 IHC (p), WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal dilution of the B3GNT8 antibody should be determined by the researcher.\. Western blot: 0.1-0.5 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
- 限制
- 仅限研究用
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- 缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
- 储存条件
- -20 °C
- 储存方法
- After reconstitution, the B3GNT8 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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- 抗原
- B3GNT8 (UDP-GlcNAc:betaGal beta-1,3-N-Acetylglucosaminyltransferase 8 (B3GNT8))
- 别名
- B3GNT8 (B3GNT8 产品)
- 别名
- B3GALT7 antibody, BGALT15 antibody, beta3Gn-T8 antibody, B3galt7 antibody, B7galt7 antibody, BC025206 antibody, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8 antibody, B3GNT8 antibody, B3gnt8 antibody
- 背景
- B3GNT8 is a galactosyltransferase involved in the synthesis of poly-N-acetyllactosamine (polyLacNAc), a linear chain of repeating LacNAc units made up of galactose (Gal) and N-acetylglucosamine (GlcNAc) with the structure (Gal-beta-1-4-GlcNAc-beta-1-3)n. By genomic sequence analysis, the B3GNT8 gene is mapped to chromosome 19q13.2. It was showed that a soluble form of B3GNT8 overexpressed by transfected HEK293 cells selectively transferred GlcNAc from UDP-GlcNAc to the nonreducing terminus of Gal-beta-1-4-GlcNAc-alpha-p-nitrophenyl phosphate and to lactoside-alpha-benzoyl. It did not utilize keratan sulfates or polylactosamine oligosaccharide as substrate. B3GNT8 activity required Mn(2+) and showed less efficiency with Co(2+). The pH optimum was between 7 and 7.5. B3GNT8 also transferred GlcNAc onto alpha-1-acid glycoprotein and ovomucoid, which possess tetraantennary complex type and pentaantennary complex type N-glycans. With a tetraantennary N-glycan substrate, B3GNT8 appeared to prefer the beta-1-2 branch over the beta-1-6 branch. When overexpressed in HCT15 human colon cancer cells, B3GNT8 increased cell surface expression of both polyLacNAc and beta-1-6-branched N-glycans.
- UniProt
- Q7Z7M8
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