G-CSF 抗体
Quick Overview for G-CSF 抗体 (ABIN4949702)
抗原
See all G-CSF (CSF3) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Human recombinant full-length protein was used as the immunogen for the G-CSF antibody.
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亚型
- IgG1
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anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 155-198) antibody
CSF3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 1-207) antibodyCSF3 适用: 人 ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 8G5F7 unconjugated
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 1-207) antibodyCSF3 适用: 人 ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 7E4F7 unconjugated
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 30-207) antibodyCSF3 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 1-207) antibodyCSF3 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) antibodyCSF3 适用: 人, Macaque IHC, StM 宿主: 小鼠 Monoclonal rCSF3-900 unconjugated Recombinant Antibody
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) antibodyCSF3 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 47-205) antibodyCSF3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 27-200) antibodyCSF3 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) antibodyCSF3 适用: 人, Macaque IHC, StM 宿主: 兔 Monoclonal CSF3-3166R unconjugated Recombinant Antibody
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应用备注
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Optimal dilution of the G-CSF antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the G-CSF antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
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别名
- G-CSF
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背景
- This mAb recognizes granulocyte-colony stimulating factor (G-CSF) in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell types. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.G-CSF is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage. The human G-CSF cDNA encodes a 207 amino acid precursor containing a 29 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSF's, which are identical except for a three amino acid deletion in the amino-terminus of one form of the protein have been isolated from human cells. Murine and human G-CSF's share 73 % sequence identity at the amino acid level.
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
抗原
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