ATG5 抗体
Quick Overview for ATG5 抗体 (ABIN492606)
抗原
See all ATG5 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes the ATG5-ATG12 protein complex at 55 kDa and ATG5 at 33 kDa in Immunoblot application.
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产品特性
- Synonyms: ASP, APG5-like, Autophagy protein 5, Apoptosis-specific protein
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纯化方法
- Purified from Serum-Free Cell Culture Supernatant by subsequent ultrafiltration and Size Exclusion Chromatography
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组件
- incl. positive Control
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免疫原
- Recombinant ATG5
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亚型
- IgG1
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anti-ATG5 Autophagy Related 5 (ATG5) (AA 101-200) antibody
ATG5 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (AA 1-275) antibodyATG5 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (AA 209-238), (C-Term) antibodyATG5 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB7547 unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (AA 144-275) antibodyATG5 适用: 人 ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 3C7A8 unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (AA 176-275) antibodyATG5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1G11 unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (AA 1-119) antibodyATG5 适用: 人 WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal ATG5-2101 unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (AA 1-30) antibodyATG5 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (AA 1-30), (N-Term) antibodyATG5 适用: 人, 小鼠 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (C-Term) antibodyATG5 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (AA 28-275) antibodyATG5 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunobotting (Western Blot): 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking buffer and blotincubation buffer. Immunocytochemistry: 0.5 μg/mL. Flow Cytometry. Included Postitive Control: Cell lysate from untreated SH-SY5Y cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- Positive Control: Cell lysate from untreated SH-SY5Y cells. Formulation: Lyophilized cell lysate from Serum starved SH-SY5Y cells. Stability: Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2xSDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The Positive Control lysate is recommended for Immunoblot applications. 20µl of PositiveControl correspond to ca. 20. 000 cells. Use 20µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Shelf life: one year from despatch. The Lyophilized cell lysates contain SDS and are not recommended for applications withnative proteins such as Immunoprecipitation.
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限制
- 仅限研究用
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溶解方式
- Restore with 1 mL H2O (15 min, RT).
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缓冲液
- PBS, 0.09 % Sodium Azide, PEG and Sucrose
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -20 °C to -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
Shelf life: one year from despatch. -
有效期
- 12 months
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- ATG5 (ATG5 Autophagy Related 5 (ATG5))
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别名
- APG5L / ATG5
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背景
- Autophagy as a response to cellular stress and starvation is an alternative process of proteasomal degradation for some long-lived proteins and organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). The gene product of autophagy-related gene 5 (ATG5) is required for autophagosome-formation. ATG5 also enhances the susceptibility towards apoptotic stimuli. Like Bcl-2, ATG5 exhibits a dual function by modulating both autophagy and apoptosis.Synonyms: APG5-like, ASP, Apoptosis-specific protein, Autophagy protein 5
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分子量
- 24, 33, 55 kDa
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基因ID
- 9474
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UniProt
- Q9H1Y0
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途径
- Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy
抗原
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