SHANK3 抗体 (AA 840-857)
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- 抗原 See all SHANK3 抗体
- SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))
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抗原表位
- AA 840-857
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适用
- 大鼠, 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This SHANK3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Detects ~190 kDa protein. No cross-reactivity against Shank1 or Shank2.
- 产品特性
- Synonyms: PROSAP2, PSAP2, SPANK-2, SPANK2, KIAA1650, SH3 and multiple ankyrin repeat domainsprotein 3, Proline-rich synapse-associated protein 2
- 纯化方法
- Protein G Chromatography
- 免疫原
- Synthetic peptide amino acids 840-857 of Rat Shank3 (accession number Q9JLU4). AA Sequence: PEKLPGSLRKGIPRTKSV
- 克隆位点
- S69-46
- 亚型
- IgG2b
- Top Product
- Discover our top product SHANK3 Primary Antibody
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anti-SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3) (AA 840-857) antibody (HRP)
SHANK3 适用: 大鼠 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal S69 HRP
anti-SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3) (AA 840-857) antibody (FITC)SHANK3 适用: 大鼠 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal S69 FITC
anti-SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3) (AA 840-857) antibody (Biotin)SHANK3 适用: 大鼠 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal S69 Biotin
anti-SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3) (AA 840-857) antibody (PE)SHANK3 适用: 大鼠 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal S69 PE
anti-SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3) (AA 840-857) antibody (APC)SHANK3 适用: 大鼠 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal S69 APC
anti-SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3) (AA 840-857) antibody (PerCP)SHANK3 适用: 大鼠 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal S69 PerCP
anti-SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3) (AA 840-857) antibody (Atto 488)SHANK3 适用: 大鼠 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal S69 Atto 488
anti-SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3) (AA 840-857) antibody (Atto 594)SHANK3 适用: 大鼠 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal S69 Atto 594
anti-SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3) (AA 840-857) antibody (Atto 390)SHANK3 适用: 大鼠 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal S69 Atto 390
anti-SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3) (AA 840-857) antibodySHANK3 适用: 大鼠 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal S69 unconjugated
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- 应用备注
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Western blot: 1 μg/mL1 μg/mL was sufficient for detection of Shank3 in 10 μg of COS cell (lysate) transientlytransfected with Shank3 by colorimetric immunoblot analysis using Goat anti-mouseIgG: HRP as the secondary antibody. Immunoprecipitation: 1.0-10 μg/mLImmunofluorescence: 1.0-10 μg/mLImmunocytochemistry: 0.1-1.0 μg/mLImmunohistochemistry: 0.1-1.0 μg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.4 containing 50 % Glycerol as stabilizer and 0.09 % Sodium Azide as preservative
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))
- 别名
- SHANK3 (SHANK3 产品)
- 别名
- DEL22q13.3 antibody, PROSAP2 antibody, PSAP2 antibody, SCZD15 antibody, SPANK-2 antibody, Prosap2 antibody, AI841104 antibody, Shank3b antibody, SH3 and multiple ankyrin repeat domains 3 antibody, SH3 and multiple ankyrin repeat domains protein 3 antibody, SHANK3 antibody, shank3 antibody, Shank3 antibody
- 背景
- Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inwardrectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins (3). Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank proteins are also crucial in receptor tyrosine kinase signaling, specifically, Shank3 can mediate Erk-MAPK and P13K signaling which is crucial for tubule formation (4). Shank3 is also one of the latest genes to be associated with autism. A mutation of a single copy of Shank3 on chromosome 22q13 can result in language and/or social communication disorders (5).Synonyms: KIAA1650, PROSAP2, PSAP2, Proline-rich synapse-associated protein 2, SH3 and multiple ankyrin repeat domains protein 3, SPANK-2, SPANK2
- 基因ID
- 59312
- UniProt
- Q9JLU4
- 途径
- Synaptic Membrane, Tube Formation, Regulation of long-term Neuronal Synaptic Plasticity
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