YAP1 抗体 (N-Term)
Quick Overview for YAP1 抗体 (N-Term) (ABIN4886772)
抗原
See all YAP1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 62-97, N-Term
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原理
- Anti-YAP1 Antibody Picoband®
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序列
- ETDLEALFNA VMNPKTANVP QTVPMRLRKL PDSFFK
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-YAP1 Antibody Picoband® (ABIN4886772). Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human YAP1, identical to the related mouse and rat sequences.
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亚型
- IgG
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anti-Yes-Associated Protein 1 (YAP1) (AA 53-161) antibody
YAP1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2F12 unconjugated
anti-Yes-Associated Protein 1 (YAP1) antibodyYAP1 适用: 人 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Yes-Associated Protein 1 (YAP1) antibodyYAP1 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 1A12 unconjugated
anti-Yes-Associated Protein 1 (YAP1) antibodyYAP1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Yes-Associated Protein 1 (YAP1) (AA 131-330) antibodyYAP1 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Yes-Associated Protein 1 (YAP1) (C-Term) antibodyYAP1 适用: 人, 小鼠, 大鼠, 斑马鱼, 犬, 绵羊, Cow, 马, Pig, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Yes-Associated Protein 1 (YAP1) (AA 53-162) antibodyYAP1 适用: 人 WB, ELISA, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 2F12 unconjugated
anti-Yes-Associated Protein 1 (YAP1) antibodyYAP1 适用: 人 WB, FACS, IHC (p), EIA 宿主: 小鼠 Monoclonal 1A12 unconjugated
anti-Yes-Associated Protein 1 (YAP1) (pSer127) antibodyYAP1 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Yes-Associated Protein 1 (YAP1) (AA 351-400) antibodyYAP1 适用: 人, 小鼠, 大鼠, 斑马鱼, 犬, 猴, 绵羊, 小鸡, Cow, 马, Pig, 兔, 非洲爪蟾, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Huang, J., Wu, S., Barrera, J., Matthews, K., Pan, D. (2005). "The Hippo signaling pathway coordinately regulates cell proliferation and apoptosis by inactivating Yorkie, the Drosophila Homolog of YAP". Cell122 (3): 421-34. 2. Overholtzer M, Zhang J, Smolen GA, Muir B, Li W, Sgroi DC, Deng CX, Brugge JS, Haber DA. (2006). "Transforming properties of YAP, a candidate oncogene on the chromosome 11q22 amplicon". Proc Natl Acad Sci U S A. 103 (33): 12405-12410. 3. Sudol M (1994). "Yes-associated protein (YAP65) is a proline-rich phosphoprotein that binds to the SH3 domain of the Yes proto-oncogene product". Oncogene 9 (8): 2145-2152. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- YAP1 (Yes-Associated Protein 1 (YAP1))
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别名
- YAP1
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背景
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Synonyms: Transcriptional coactivator YAP1,Yes-associated protein 1,Protein yorkie homolog,Yes-associated protein YAP65 homolog,YAP1,YAP65,
Tissue Specificity: Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level). .
Background: YAP1, also known as YAP or YAP65, is a potent oncogene, which is amplified in various human cancers. This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. It is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.
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分子量
- 75 kDa
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基因ID
- 10413
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UniProt
- P46937
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途径
- MAPK Pathway, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
抗原
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