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Aconitase 1 抗体 (C-Term, N-Term)

Name: Aconitase 1 抗体 (C-Term, N-Term)
SKU: ABIN488534
Availability: OutOfStock

ACO1 适用: Arabidopsis thaliana, Tomato WB 宿主: 兔 Polyclonal unconjugated

Image no. 1 for anti-Aconitase 1 (ACO1) (C-Term), (N-Term) antibody (ABIN488534) (Aconitase 1 抗体 (C-Term, N-Term))

1 image

(1 reference)

产品编号 ABIN488534

发货至: 中国

Datasheet as PDF

抗原 See all Aconitase 1 (ACO1) 抗体

Aconitase 1 (ACO1)
抗原表位
20

18

5

4

3

3

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term, N-Term
适用
101

45

40

3

2

2

1

1

1

1

1

1

1
Arabidopsis thaliana, Tomato
宿主
104

3

1
兔
Compatible Secondaries
克隆类型
106

2
多克隆
标记
50

12

8

4

3

3

3

3

3

3

3

3

3

3

3

1
This Aconitase 1 antibody is un-conjugated
应用范围
94

39

34

27

26

20

11

11

9

4

3

2
Western Blotting (WB)

交叉反应（详细）

Not reactive in: Chlamydomonas reinhardii

预测反应

dicots including:Cucurbita maxima, Nicotiana tabacum, Ricinus communis, Solanum tuberosum, Vitis vinifera,monocots: Oryza sativa, Zea mays, trees: Picea sitchensis, Populus trichocarpa

产品特性

Expected / apparent Molecular Weight of the Antigene: 98 kDa. Note that ACO1, ACO2 and ACO3 cannot be distinguished in size by standard SDS-PAGE.
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA, immunoblotting and enzyme inhibition.

纯化方法

serum

免疫原

Arabidopsis ACO1 (AT4G35830, Q42560), codon 120 - 898 (C-terminus), was cloned in fusion with a N-terminal 6xHis tag, and over-expressed in E. coli. All recombinant protein accumulated in inclusion bodies, which were purified by centrifugation and solubilised in 6 M guanidine-HCl. The protein was refolded by dilution in 100 mM Tris-HCl 8.5, 10% (v/v) glycerol, 2 mM dithiothreitol, and concentrated prior to immunisation.

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ACO1 适用: 小鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

1:5 000 - 1:10 000 with standard ECL (WB). At higher concentrations the antibody binds aspecifically resulting in non-specific signals âper thousand 60 kDa, including Rubisco subunits.

说明

The antibody recognises all three Arabidopsis aconitase isoforms (ACO1, ACO2 and ACO3, see Bernard et al 2009). Possible differences in affinity have not been precisely quantified. Sensitivity threshold is between 2 and 10 ng for WB / ECL (see figure). Antibodies will recognize aconitase isoforms in denaturing and native gel electrophoresis.

限制

仅限研究用
状态

Lyophilized

溶解方式

For reconstitution add 100 μL of sterile water.

注意事项

Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

储存条件

-20 °C

储存方法

store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).
抗原

Aconitase 1 (ACO1)

别名

ACO1 (ACO1 产品)

别名

ACONS antibody, IREB1 antibody, IREBP antibody, IREBP1 antibody, IRP1 antibody, Acon1 antibody, Ratireb antibody, ratireb antibody, Aco1 antibody, AI256519 antibody, Aco-1 antibody, Irebp antibody, Irp1 antibody, aconitase 1 antibody, aconitase 1, soluble antibody, aconitase 1 L homeolog antibody, ACO1 antibody, Aco1 antibody, aco1.L antibody

背景

AGI Code: At4g35830
Aconitase is a single subunit enzyme of the tricarboxylic acid cycle (or Krebs cycle) in the mitochondria. A cytosolic isoform is also part of the glyoxylate cycle. Aconitase catalyzes the dehydration / hydration of citrate to iso-citrate, via cis-aconitate as an intermediate. The reaction is facilitated by an iron-sulphur cluster in the active site of the enzyme. The iron-sulphur cluster is somewhat unstable, especially under oxidative stress, and loss of the cofactor leads to degradation of the protein.Alternative names: ACO, citrate hydro-lyase 1,2,3

分子量

98 kDa. Note that ACO1 kDa, ACO2 and ACO3 cannot be distinguished in size by standard SDS-PAGE.

UniProt

Q42560

途径

Transition Metal Ion Homeostasis