DIAPH1 抗体 (Internal Region)
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- 抗原 See all DIAPH1 抗体
- DIAPH1 (Diaphanous Homolog 1 (DIAPH1))
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抗原表位
- Internal Region
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适用
- 人, 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This DIAPH1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- This antibody is expected to recognize both reported isoforms (NP_005210.3 and NP_001073280.1).
- 交叉反应 （详细）
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Species reactivity (expected):Human.
Species reactivity (tested):Mouse. - 纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
- 免疫原
- Peptide with sequence C-QTKTSKAKKDQ, from the internal region of the protein sequence according to NP_005210.3 and NP_001073280.1. Genename: DIAPH1
- Top Product
- Discover our top product DIAPH1 Primary Antibody
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anti-Diaphanous Homolog 1 (DIAPH1) (C-Term) antibody
DIAPH1 适用: 人, 小鼠 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Diaphanous Homolog 1 (DIAPH1) antibodyDIAPH1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Diaphanous Homolog 1 (DIAPH1) (N-Term) antibodyDIAPH1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Diaphanous Homolog 1 (DIAPH1) (AA 1-404) antibodyDIAPH1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Diaphanous Homolog 1 (DIAPH1) antibodyDIAPH1 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Diaphanous Homolog 1 (DIAPH1) (AA 389-583) antibodyDIAPH1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Diaphanous Homolog 1 (DIAPH1) (AA 18-52), (N-Term) antibodyDIAPH1 适用: 人 WB 宿主: 兔 Polyclonal RB30760 unconjugated
anti-Diaphanous Homolog 1 (DIAPH1) (AA 921-1024) antibodyDIAPH1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Diaphanous Homolog 1 (DIAPH1) (AA 1-404) antibodyDIAPH1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Diaphanous Homolog 1 (DIAPH1) (AA 1-404) antibodyDIAPH1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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- 应用备注
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Peptide ELISA: Detection Limit Dilution: 1/32000. Western blot: 0.3-1 μg/mL. Approx 140 kDa band observed in lysates of cell line NIH3T3(calculated MW of 140 kDa according to Human NP_005210.3 and 130 kDa according toMouse NP_031884.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- 抗原
- DIAPH1 (Diaphanous Homolog 1 (DIAPH1))
- 别名
- DIAPH1 (DIAPH1 产品)
- 别名
- DIAPH1 antibody, dia1 antibody, drf1 antibody, dfna1 antibody, diap1 antibody, lfhl1 antibody, DIAPH2 antibody, Diap1 antibody, D18Wsu154e antibody, Dia1 antibody, Diaph1 antibody, Drf1 antibody, mKIAA4062 antibody, p140mDia antibody, DFNA1 antibody, DIA1 antibody, DRF1 antibody, LFHL1 antibody, hDIA1 antibody, diaphanous related formin 1 antibody, diaphanous related formin 2 antibody, diaphanous-related formin 1 antibody, protein diaphanous homolog 1 antibody, DIAPH1 antibody, diaph1 antibody, DIAPH2 antibody, Diaph1 antibody, LOC100457442 antibody
- 背景
- DIAPH1 binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (By similarity). In hearing it may play a role in the regulation of actin polymerization in hair cells.Synonyms: DIAP1, DRF1, Diaphanous homolog 1, Diaphanous-1, Diaphanous-related formin-1
- 基因ID
- 1729
- NCBI登录号
- NP_005210
- UniProt
- O60610
- 途径
- Sensory Perception of Sound
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