Adenosine A2a Receptor 抗体
-
- 抗原 See all Adenosine A2a Receptor (ADORA2A) 抗体
- Adenosine A2a Receptor (ADORA2A)
-
适用
- 人, 小鼠, 大鼠, 犬, 兔, 豚鼠
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This Adenosine A2a Receptor antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- This antibody recognizes Human Adenosine Receptor A2A at ~43 kDa. Does not recognize Adenosine Receptor A2A in knockout mice.
- 交叉反应 (详细)
-
Species reactivity (expected):Mouse, Rat, Canine (Dog), Guinea Pig and Rabbit.
Species reactivity (tested):Human. - 产品特性
- Synonyms: ADORA2A, ADORA2
- 纯化方法
- Protein G Chromatography
- 免疫原
- 6His-tagged full-length Human recombinant A2A receptor. Genename: ADORA2ARemarks: Epitope mapped to SQPLPGER in the third intracellular loop.
- 克隆位点
- N-n
- 亚型
- IgG2a
- Top Product
- Discover our top product ADORA2A Primary Antibody
-
-
- 应用备注
-
Western blot: 1/1000-1/5000. Flow Cytometry. Immunohistochemistry (Paraffin Sections): 5 μg/mL. Immunohistochemistry (4 % Paraformaldehyde): 1 μg/mL. This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
-
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.01 M Tris-Glycine, pH 7.4, 0.11 M sodium chloride, 0.05 % sodium azide.
- 储存条件
- 4 °C/-20 °C
- 储存方法
-
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
-
- 抗原
- Adenosine A2a Receptor (ADORA2A)
- 别名
- Adenosine Receptor A2a (ADORA2A 产品)
- 背景
- The A2a Adenosine Receptor is a major target of caffeine. This receptor inhibits cell aggregation, induces vasodilation, and downregulates inflammation. This gene encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.Synonyms: ADORA2, ADORA2A
- 基因ID
- 135
- UniProt
- P29274
- 途径
- Neurotrophin Signaling Pathway, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints
-