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Adenosine A2a Receptor 抗体

This anti-Adenosine A2a Receptor antibody is a 小鼠 单克隆 antibody detecting Adenosine A2a Receptor in WB, FACS 和 IHC (p). Suitable for 人, 小鼠, 大鼠, 犬, 兔 和 豚鼠.
产品编号 ABIN487435
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Adenosine A2a Receptor 抗体 (ABIN487435)

抗原

See all Adenosine A2a Receptor (ADORA2A) 抗体
Adenosine A2a Receptor (ADORA2A)

适用

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人, 小鼠, 大鼠, 犬, 兔, 豚鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This Adenosine A2a Receptor antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

N-n
  • 特异性

    This antibody recognizes Human Adenosine Receptor A2A at ~43 kDa. Does not recognize Adenosine Receptor A2A in knockout mice.

    交叉反应 (详细)

    Species reactivity (expected):Mouse, Rat, Canine (Dog), Guinea Pig and Rabbit.
    Species reactivity (tested):Human.

    产品特性

    Synonyms: ADORA2A, ADORA2

    纯化方法

    Protein G Chromatography

    免疫原

    6His-tagged full-length Human recombinant A2A receptor. Genename: ADORA2ARemarks: Epitope mapped to SQPLPGER in the third intracellular loop.

    亚型

    IgG2a
  • 应用备注

    Western blot: 1/1000-1/5000. Flow Cytometry. Immunohistochemistry (Paraffin Sections): 5 μg/mL. Immunohistochemistry (4 % Paraformaldehyde): 1 μg/mL. This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    0.01 M Tris-Glycine, pH 7.4, 0.11 M sodium chloride, 0.05 % sodium azide.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
    Shelf life: one year from despatch.

    有效期

    12 months
  • 抗原

    Adenosine A2a Receptor (ADORA2A)

    别名

    Adenosine Receptor A2a

    背景

    The A2a Adenosine Receptor is a major target of caffeine. This receptor inhibits cell aggregation, induces vasodilation, and downregulates inflammation. This gene encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.Synonyms: ADORA2, ADORA2A

    基因ID

    135

    UniProt

    P29274

    途径

    Neurotrophin Signaling Pathway, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints
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