Adenosine A2a Receptor 抗体
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Quick Overview for Adenosine A2a Receptor 抗体 (ABIN487435)
抗原
See all Adenosine A2a Receptor (ADORA2A) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody recognizes Human Adenosine Receptor A2A at ~43 kDa. Does not recognize Adenosine Receptor A2A in knockout mice.
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交叉反应 (详细)
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Species reactivity (expected):Mouse, Rat, Canine (Dog), Guinea Pig and Rabbit.
Species reactivity (tested):Human. -
产品特性
- Synonyms: ADORA2A, ADORA2
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纯化方法
- Protein G Chromatography
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免疫原
- 6His-tagged full-length Human recombinant A2A receptor. Genename: ADORA2ARemarks: Epitope mapped to SQPLPGER in the third intracellular loop.
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亚型
- IgG2a
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应用备注
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Western blot: 1/1000-1/5000. Flow Cytometry. Immunohistochemistry (Paraffin Sections): 5 μg/mL. Immunohistochemistry (4 % Paraformaldehyde): 1 μg/mL. This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- 0.01 M Tris-Glycine, pH 7.4, 0.11 M sodium chloride, 0.05 % sodium azide.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- Adenosine A2a Receptor (ADORA2A)
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别名
- Adenosine Receptor A2a
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背景
- The A2a Adenosine Receptor is a major target of caffeine. This receptor inhibits cell aggregation, induces vasodilation, and downregulates inflammation. This gene encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.Synonyms: ADORA2, ADORA2A
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基因ID
- 135
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UniProt
- P29274
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途径
- Neurotrophin Signaling Pathway, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints
抗原
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