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Adenosine A2a Receptor 抗体

ADORA2A 适用: 人, 小鼠, 大鼠, 犬, 兔, 豚鼠 WB, FACS, IHC (p) 宿主: 小鼠 Monoclonal n-n unconjugated
产品编号 ABIN487435
发货至: 中国
  • 抗原 See all Adenosine A2a Receptor (ADORA2A) 抗体
    Adenosine A2a Receptor (ADORA2A)
    适用
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    人, 小鼠, 大鼠, 犬, 兔, 豚鼠
    宿主
    • 68
    • 10
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    小鼠
    克隆类型
    • 72
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    • 1
    单克隆
    标记
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    This Adenosine A2a Receptor antibody is un-conjugated
    应用范围
    • 61
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    Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    特异性
    This antibody recognizes Human Adenosine Receptor A2A at ~43 kDa. Does not recognize Adenosine Receptor A2A in knockout mice.
    交叉反应 (详细)
    Species reactivity (expected):Mouse, Rat, Canine (Dog), Guinea Pig and Rabbit.
    Species reactivity (tested):Human.
    产品特性
    Synonyms: ADORA2A, ADORA2
    纯化方法
    Protein G Chromatography
    免疫原
    6His-tagged full-length Human recombinant A2A receptor. Genename: ADORA2ARemarks: Epitope mapped to SQPLPGER in the third intracellular loop.
    克隆位点
    N-n
    亚型
    IgG2a
    Top Product
    Discover our top product ADORA2A Primary Antibody
  • 应用备注
    Western blot: 1/1000-1/5000. Flow Cytometry. Immunohistochemistry (Paraffin Sections): 5 μg/mL. Immunohistochemistry (4 % Paraformaldehyde): 1 μg/mL. This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    0.01 M Tris-Glycine, pH 7.4, 0.11 M sodium chloride, 0.05 % sodium azide.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
    Shelf life: one year from despatch.
    有效期
    12 months
  • 抗原
    Adenosine A2a Receptor (ADORA2A)
    别名
    Adenosine Receptor A2a (ADORA2A 产品)
    背景
    The A2a Adenosine Receptor is a major target of caffeine. This receptor inhibits cell aggregation, induces vasodilation, and downregulates inflammation. This gene encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.Synonyms: ADORA2, ADORA2A
    基因ID
    135
    UniProt
    P29274
    途径
    Neurotrophin Signaling Pathway, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints
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